评价晚期结直肠癌患者CRNDE血浆表达水平、KRAS、NRAS和BRAF变异之间的关系

IF 0.3 4区医学 Q4 ONCOLOGY

Uhod-Uluslararasi Hematoloji-Onkoloji Dergisi Pub Date : 2023-06-30 DOI:10.4999/uhod.236797

Serap Arslan

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引用次数: 0

摘要

结直肠癌是最常见的癌症之一。CRNDE是一个新定义的lncRNA。我们评估了使用CRDNE循环lncRNA作为非侵入性生物标志物的可能性。此外，我们还研究了循环CRNDE lncRNA对CRC发病机制的影响，以及它与CRC中常见的KRAS、NRAS和BRAF体细胞变异的关系。在本研究中，我们招募了50例晚期结直肠癌患者的血浆和FFPE组织样本，以及31例年龄相近的对照组的血浆样本。然后，我们分别进行血浆提取、总RNA分离、cDNA合成和循环lncRNA表达分析。此外，我们还对FFPE组织进行了KRAS、NRAS和BRAF体细胞变异分析。我们的研究结果显示，与对照组相比，CRC中CRNDE的表达水平显著上调(p= 0.002)。计算得到受者工作特性曲线下面积为0.70。我们发现KRAS体细胞变异与循环CRNDE lncRNA之间存在统计学差异(p= 0.031)。本研究表明，CRNDE循环lncrna可作为CRC的潜在非侵入性生物标志物。在我们的研究中，我们确定了在晚期CRC病例中频繁观察到的KRAS体细胞变异与CRNDE之间存在显著的关系。据我们所知，这是第一个显示CRNDE循环lncRNA与晚期结直肠癌相关的研究。此外，该研究首次揭示了晚期结直肠癌中循环CRNDE lncRNA之间的KRAS体细胞变异之间的关系。关键词:CRNDE，循环lncRNA, KRAS, CRC, RT-PCR

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Evaluation of the Association Between CRNDE Plasma Expression Level, KRAS, NRAS, and BRAF Variants in Patients with advanced CRC

Colorectal cancer is one of the most common types of cancer. CRNDE is a novel-defined lncRNA. We evaluated the possibility of using CRDNE circulating lncRNA as a noninvasive biomarker. Also, we examined the effect of circulating CRNDE lncRNA on the pathogenesis of CRC and its association with the KRAS, NRAS, and BRAF somatic variants commonly observed in CRC. In this study, we enrolled plasma and FFPE tissue samples of 50 advanced CRC patients and plasma samples of 31 individuals in the control group of similar ages. Then, we performed plasma extraction, total RNA isolation, cDNA synthesis, and circulating lncRNA expression analysis, respectively. Also, we carried out KRAS, NRAS, and BRAF somatic variant analysis from FFPE tissues. Our results showed that the expression level of CRNDE (p= 0.002) were significantly upregulated in the CRC when it was compared to with the control group. The calculated area under the curve of the receiver operating characteristic was 0.70. We found a statistically significant difference between the KRAS somatic variants and the circulating CRNDE lncRNA (p= 0.031). This study demonstrated that CRNDE circulating lncRNAs may be used as a potential non-invasive biomarker in CRC. In our study, it was determined that there is a significant relationship between the frequently observed KRAS somatic variants and CRNDE in advanced-stage CRC cases. To the best of our knowledge, this is the first study to show the association of CRNDE circulating lncRNA with advanced CRC. Moreover, it is the first study to show a relationship between the KRAS somatic variants between circulating CRNDE lncRNA in advanced CRC. Keywords: CRNDE, Circulating lncRNA, KRAS, CRC, RT-PCR

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来源期刊

Uhod-Uluslararasi Hematoloji-Onkoloji Dergisi ONCOLOGY-

CiteScore

1.60

自引率

16.70%

发文量

审稿时长

>12 weeks

期刊介绍： Target of Publication is the public representation of new scientific results of fundamental, problem-oriented, and search scientific investigations; analytical articles and reviews by actual scientific problems of coal mining industry, short reports in the field of coal mining industry, including the results of work of scientific meetings, conferences, summit’s and congresses in the field of mining. The basic purpose of the journal is the promotion of innovative activity in the coal mining industry by highlighting and discussing the results of basic and applied research, the experience in applying results of innovation for the development of the Russian coal mining. The most important tasks of the journal are: the generalization of scientific and practical achievements of specialists in the field of coal mining industry, specializing in the implementation of promising basic and applied research and development of priorities of high technologies; the scientific concept of the publication; the publication of the latest achievements in the field of innovation in coal mining industry. The journal lays special emphasis on methodical elaborations, analytical investigations in the sphere of coal mining. Targets of Journal are in publication of new results of scientific investigations performed by scientists, post-graduate students, and doctoral candidates performed by from higher schools, Russian Academy of Sciences, other scientific organizations of Russia, CIS countries, and foreign universities