盆腔脱垂患者阴道组织的免疫组化年龄变化

Q4 Medicine
R.A. Safonov, V.Y. Prokopiuk, O.V. Hryshchenko, O.S. Prokopiuk, V.V. Lazurenko, M.H. Hryshchenko, V.Y. Parashchuk
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引用次数: 0

摘要

研究目的:研究盆腔脱垂(PP)女性阴道组织结构的年龄相关性变化。材料和方法。对29例PP女性阴道壁结构进行了研究,将她们分为三组:第一组——11名45岁、月经功能正常的育龄期女性;第二组为8例45 ~ 55岁围绝经期月经不规律妇女;第三组是10名年龄在55-65岁之间、绝经3年以上的女性。所有女性均行免疫组化检测CD34标志物及血管内皮生长因子(VEGF)、足素(podoplanin)、雌激素受体数量、1型基质金属蛋白酶(1型胶原酶)和1型胶原蛋白。生殖年龄的特点是代谢活跃,这反映在阴道各层丰满、增厚、细胞核异色、结缔组织代谢增加和血管生成刺激物的合成。雌激素受体的表达并没有因为体内有足够的雌激素浓度而增加。围绝经期的萎缩性变化被确定为阴道层变薄、硬化变化和以核色素过多形式的蛋白质合成减少。雌激素受体的数量因缺乏而代偿性增加。这个年龄段的一个特征是血管失衡,主观上表现为更年期症状。在绝经后,观察到萎缩和硬化的变化,表现为阴道壁变薄,核染深,胶原蛋白水平相对于胶原酶水平升高,淋巴引流减少,雌激素受体数量增加。本研究表明,所有PP组的女性都有与年龄变化、月经周期特征和身体激素饱和度相关的特征性体征。影响PP发展的因素包括:育龄期胶原酶活性增加,围绝经期和绝经后组织萎缩、性激素失调和循环障碍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunohistochemical age changes of vagina tissues in women with pelvic prolaps
Research objectives: to study age-related changes in the structure of vaginal tissues in women with pelvic prolapse (PP).Materials and methods. The structure of the vaginal wall was studied in 29 women with PP, who were divided into three groups: the first group – 11 women aged of 45 in the reproductive period with regular menstrual function; the second group – 8 women aged 45–55 in perimenopause with irregular menstrual function; the third group – 10 women aged 55–65 who have been postmenopausal for more than three years.All women underwent immunohistochemical detection of the CD34 marker and vascular endothelial growth factor (VEGF), podoplanin, the number of estrogen receptors, type 1 matrix metalloproteinase (type 1 collagenase) and type 1 collagen were determined.Results. The reproductive age was characterized by an active metabolism, which was reflected in the fullness of all vaginal layers, their thickening, heterochromia of the nuclei, increased metabolism in the connective tissue and synthesis of vasculogenesis stimulators. The expression of estrogen receptors was not increased due to a sufficient concentration of estrogens in the body.Atrophic changes in the perimenopausal age were determined as the thinning of the vaginal layers, sclerotic changes, and a decrease in protein synthesis in the form of nuclear hyperchromia. The number of estrogen receptors was compensatory increased due to their deficiency. A feature of this age is vascular imbalance, which was subjectively expressed in climacteric symptoms.Atrophic and sclerotic changes were observed in the postmenopausal period in the form of thinning of the vaginal wall, hyperchromia of the nuclei, an increase in the collagen level relative to an unchanged collagenase level, a decrease in lymphatic drainage, and an increase in the number of estrogen receptors.Conclusions. This study shows that all groups of women with PP have characteristic signs associated with age changes, features of the mestral cycle and hormonal saturation of the body. Factors affecting the PP development include: in reproductive age – increased collagenase activity, in perimenopause and postmenopause – atrophic dyshormonal and dyscirculatory processes in tissues.
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来源期刊
Reproductive Endocrinology
Reproductive Endocrinology Medicine-Obstetrics and Gynecology
CiteScore
0.50
自引率
0.00%
发文量
13
审稿时长
8 weeks
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