[毛癣菌的形态和组织培养特性]。

Polskie archiwum weterynaryjne Pub Date : 1989-01-01
K Kostro
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引用次数: 0

摘要

在研究中发现,培养基的含量决定了马蹄莲菌落的生长和外观。在Sabouraud固体培养基上观察到两种类型的菌落,但以中心区具有典型的径向褶皱和凹槽的菌落和中心脐状凸起的菌落为主。作为马蹄草的典型特征,在含葡萄糖的固体Sabouraud培养基上培养时,可以接受菌落背面的暗红色色素沉着,在不含葡萄糖的相同培养基上可以接受黄色和橙色色素沉着。失去了产生特有的深红色色素能力的马孢杆菌菌株在有土壤的培养基上传几代或在实验动物身上传一代后就恢复了这种能力。没有一株马瘟菌在不含维生素的酪胺酸培养基上生长,而在含有烟酸的培养基上生长迅速。研究用的马瘟菌迅速适应于Sabouraud液体培养基,这使得获得足够数量的菌丝体用于研究++致病性和抗原结构成为可能。根据在固体培养基上的生长情况和在液体培养基上获得的菌丝体质量的观察,确定了马蹄铁的最佳培养温度为26 ~ 28℃。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Morphological and tissue-culture properties of Trichophyton equinum].

In the studies it was revealed that the content of the medium determines growth and appearance of T. equinum colony. On solid Sabouraud media two types of colonies were observed, however, colonies with characteristic radial folds and grooves in the paracentral zone and umbilical elevation in the centre dominated. As typical features of T. equinum one can accept dark red pigmentation of the reverse side of colony in the culture on solid Sabouraud medium with glucose and yellow and orange pigmentation on the same medium with no glucose. T. equinum strains which have lost the ability of creating characteristic dark red pigment regain that ability after a few passages on media with soil or after just one passage on laboratory animals. None of the studied T. equinum strains grew on medium with casamino acids free of vitamins, while they revealed rapid growth on the same medium with nicotinic acid. T. equinum strains which were used for the studies rapidly adopted to liquid Sabouraud medium and this made it possible to obtain sufficient quantity of mycelium for studies on ++pathogenicity and antigenic structure. On the basis of observations of growth on solid media and mass of mycelium gained on liquid media it was decided that the optimal temperature of incubation of T. equinum is 26-28 degrees C.

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