利用高压静水处理和超声提高混合营养培养中小球藻的还原糖产量和提取

IF 2 4区 生物学 Q2 MARINE & FRESHWATER BIOLOGY
Sibel Uzuner, Sebnem Kurhan, Gulsun Akdemir Evrendilek
{"title":"利用高压静水处理和超声提高混合营养培养中小球藻的还原糖产量和提取","authors":"Sibel Uzuner, Sebnem Kurhan, Gulsun Akdemir Evrendilek","doi":"10.1080/09670262.2023.2248216","DOIUrl":null,"url":null,"abstract":"ABSTRACTAlthough extraction of polysaccharides to convert reducing sugars (RS) from microalgae by acid or alkali pretreatments and enzymatic hydrolysis has been extensively studied, few reports exploring the use of high hydrostatic pressure processing (HHP) and ultrasonication (US) as emerging technologies for the extraction of sugars from microalgae biomass exist. Thus, the present study was conducted to determine the effects of mixotrophic growth and stress conditions (NaNO3 and CO2 concentration and light intensity) on RS and protein accumulation in the unicellular green alga Chlorella vulgaris in addition to optimization of the effectiveness of the sequential applications of HHP and US with dilute acid as well as simultaneous enzymatic saccharification on the production of RS from microalga cells. High light intensity, high CO2 concentration and limited nitrogen concentration promoted RS production. The maximum protein content (0.0683 mg g‒1) was achieved at 0.3 g l‒1 NaNO3 concentration, 7000 μmol photons m‒2 s‒1 and 6 l min‒1 CO2 concentration. The highest RS content of C. vulgaris after 48 h enzymatic saccharification (583.86 ± 13.23 mg g‒1) was obtained at 1% (w/w) acid concentration and 80% amplitude for 30 min with 79.4% RS yield. Combined US-assisted dilute acid pretreatment and enzymatic hydrolysis were also found to be more effective than HHP assisted dilute acid pretreatment and enzymatic saccharification. Therefore, microalgal biomass can be considered a suitable renewable feedstock used in fermentation.Highlights The cultivation period of Chlorella vulgaris was reduced from 25 days to 14 days using mixotrophic growing conditions.Mixotrophic conditions enhanced reducing sugar productivity.Novel extraction techniques enhanced the extraction of reducing sugar from microalgae.KEYWORDS: High hydrostatic pressure processinglight intensitymicroalgamixotrophic cultivationnitrogen starvationultrasonication AcknowledgementsThe authors would like to thank the Innovative Food Technologies Development Application and Research Center (YENIGIDAM) of Bolu Abant Izzet Baysal University (BAIBU) for HHP analyses, and I. Isci for his help with construction of the lab-scale photobioreactor.Disclosure statementNo potential conflict of interest was reported by the author(s).Author contributionsS. Uzuner: conducted a research and investigation process, specifically performed the experiments, or data/evidence collection, formal analysis (application of statistical or mathematical techniques to analyze or synthesize study data), wrote original draft (preparation and/or creation of the published work, specifically writing the initial draft); G.A. Evrendilek: specifically performed the experiments, or data/evidence collection, wrote review and edited specifically critical review, commentary or revision-including pre- or post-publication stages; S. Kurhan: performed the experiments, or data/evidence collection.Additional informationFundingThis work was supported by Republic of Turkey Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies under a project [TAGEM/16/AR-GE/36].","PeriodicalId":12032,"journal":{"name":"European Journal of Phycology","volume":"147 1","pages":"0"},"PeriodicalIF":2.0000,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced reducing sugar production and extraction for <i>Chlorella vulgaris</i> in mixotrophic cultivation using high hydrostatic pressure processing and ultrasound\",\"authors\":\"Sibel Uzuner, Sebnem Kurhan, Gulsun Akdemir Evrendilek\",\"doi\":\"10.1080/09670262.2023.2248216\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACTAlthough extraction of polysaccharides to convert reducing sugars (RS) from microalgae by acid or alkali pretreatments and enzymatic hydrolysis has been extensively studied, few reports exploring the use of high hydrostatic pressure processing (HHP) and ultrasonication (US) as emerging technologies for the extraction of sugars from microalgae biomass exist. Thus, the present study was conducted to determine the effects of mixotrophic growth and stress conditions (NaNO3 and CO2 concentration and light intensity) on RS and protein accumulation in the unicellular green alga Chlorella vulgaris in addition to optimization of the effectiveness of the sequential applications of HHP and US with dilute acid as well as simultaneous enzymatic saccharification on the production of RS from microalga cells. High light intensity, high CO2 concentration and limited nitrogen concentration promoted RS production. The maximum protein content (0.0683 mg g‒1) was achieved at 0.3 g l‒1 NaNO3 concentration, 7000 μmol photons m‒2 s‒1 and 6 l min‒1 CO2 concentration. The highest RS content of C. vulgaris after 48 h enzymatic saccharification (583.86 ± 13.23 mg g‒1) was obtained at 1% (w/w) acid concentration and 80% amplitude for 30 min with 79.4% RS yield. Combined US-assisted dilute acid pretreatment and enzymatic hydrolysis were also found to be more effective than HHP assisted dilute acid pretreatment and enzymatic saccharification. Therefore, microalgal biomass can be considered a suitable renewable feedstock used in fermentation.Highlights The cultivation period of Chlorella vulgaris was reduced from 25 days to 14 days using mixotrophic growing conditions.Mixotrophic conditions enhanced reducing sugar productivity.Novel extraction techniques enhanced the extraction of reducing sugar from microalgae.KEYWORDS: High hydrostatic pressure processinglight intensitymicroalgamixotrophic cultivationnitrogen starvationultrasonication AcknowledgementsThe authors would like to thank the Innovative Food Technologies Development Application and Research Center (YENIGIDAM) of Bolu Abant Izzet Baysal University (BAIBU) for HHP analyses, and I. Isci for his help with construction of the lab-scale photobioreactor.Disclosure statementNo potential conflict of interest was reported by the author(s).Author contributionsS. Uzuner: conducted a research and investigation process, specifically performed the experiments, or data/evidence collection, formal analysis (application of statistical or mathematical techniques to analyze or synthesize study data), wrote original draft (preparation and/or creation of the published work, specifically writing the initial draft); G.A. Evrendilek: specifically performed the experiments, or data/evidence collection, wrote review and edited specifically critical review, commentary or revision-including pre- or post-publication stages; S. Kurhan: performed the experiments, or data/evidence collection.Additional informationFundingThis work was supported by Republic of Turkey Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies under a project [TAGEM/16/AR-GE/36].\",\"PeriodicalId\":12032,\"journal\":{\"name\":\"European Journal of Phycology\",\"volume\":\"147 1\",\"pages\":\"0\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2023-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Journal of Phycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/09670262.2023.2248216\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MARINE & FRESHWATER BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Phycology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/09670262.2023.2248216","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

虽然通过酸或碱预处理和酶水解从微藻中提取多糖转化还原糖(RS)已经得到了广泛的研究,但利用高压流体静压处理(HHP)和超声波(US)作为从微藻生物质中提取糖的新兴技术的报道很少。因此,本研究旨在确定混合营养生长和胁迫条件(NaNO3、CO2浓度和光照强度)对单细胞绿藻小球藻RS和蛋白质积累的影响,并优化HHP和US与稀酸顺序施用以及同时酶糖化对微藻细胞RS生产的有效性。高光强、高CO2浓度和有限氮浓度促进了RS的生产。在0.3 g l - 1 NaNO3浓度、7000 μmol光子m-2 s-1和6 l min-1 CO2浓度下,蛋白质含量最高,为0.0683 mg g - 1。在1% (w/w)酸浓度、80%振幅条件下,发酵30 min,发酵48 h后粗糖含量最高(583.86±13.23 mg g-1),粗糖收率为79.4%。与HHP辅助的稀酸预处理和酶解相比,us辅助的稀酸预处理和酶解的效果更好。因此,微藻生物量可以被认为是一种适合用于发酵的可再生原料。在混合营养条件下,普通小球藻的培养周期由25天缩短为14天。混合营养条件提高了还原糖产量。新的提取技术提高了微藻中还原糖的提取效率。关键词:高压静水处理;光强;微藻异营养培养;;氮饥饿;披露声明作者未报告潜在的利益冲突。作者的贡献。Uzuner:进行研究和调查过程,具体进行实验,或数据/证据收集,形式分析(应用统计或数学技术分析或综合研究数据),撰写初稿(准备和/或创作已发表的作品,具体撰写初稿);G.A. Evrendilek:专门进行实验或数据/证据收集,撰写评论和编辑特别重要的评论,评论或修订-包括出版前或出版后阶段;S. Kurhan:进行实验,或数据/证据收集。本研究得到了土耳其共和国农林部农业研究与政策总局在一个项目[TAGEM/16/AR-GE/36]下的支持。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhanced reducing sugar production and extraction for Chlorella vulgaris in mixotrophic cultivation using high hydrostatic pressure processing and ultrasound
ABSTRACTAlthough extraction of polysaccharides to convert reducing sugars (RS) from microalgae by acid or alkali pretreatments and enzymatic hydrolysis has been extensively studied, few reports exploring the use of high hydrostatic pressure processing (HHP) and ultrasonication (US) as emerging technologies for the extraction of sugars from microalgae biomass exist. Thus, the present study was conducted to determine the effects of mixotrophic growth and stress conditions (NaNO3 and CO2 concentration and light intensity) on RS and protein accumulation in the unicellular green alga Chlorella vulgaris in addition to optimization of the effectiveness of the sequential applications of HHP and US with dilute acid as well as simultaneous enzymatic saccharification on the production of RS from microalga cells. High light intensity, high CO2 concentration and limited nitrogen concentration promoted RS production. The maximum protein content (0.0683 mg g‒1) was achieved at 0.3 g l‒1 NaNO3 concentration, 7000 μmol photons m‒2 s‒1 and 6 l min‒1 CO2 concentration. The highest RS content of C. vulgaris after 48 h enzymatic saccharification (583.86 ± 13.23 mg g‒1) was obtained at 1% (w/w) acid concentration and 80% amplitude for 30 min with 79.4% RS yield. Combined US-assisted dilute acid pretreatment and enzymatic hydrolysis were also found to be more effective than HHP assisted dilute acid pretreatment and enzymatic saccharification. Therefore, microalgal biomass can be considered a suitable renewable feedstock used in fermentation.Highlights The cultivation period of Chlorella vulgaris was reduced from 25 days to 14 days using mixotrophic growing conditions.Mixotrophic conditions enhanced reducing sugar productivity.Novel extraction techniques enhanced the extraction of reducing sugar from microalgae.KEYWORDS: High hydrostatic pressure processinglight intensitymicroalgamixotrophic cultivationnitrogen starvationultrasonication AcknowledgementsThe authors would like to thank the Innovative Food Technologies Development Application and Research Center (YENIGIDAM) of Bolu Abant Izzet Baysal University (BAIBU) for HHP analyses, and I. Isci for his help with construction of the lab-scale photobioreactor.Disclosure statementNo potential conflict of interest was reported by the author(s).Author contributionsS. Uzuner: conducted a research and investigation process, specifically performed the experiments, or data/evidence collection, formal analysis (application of statistical or mathematical techniques to analyze or synthesize study data), wrote original draft (preparation and/or creation of the published work, specifically writing the initial draft); G.A. Evrendilek: specifically performed the experiments, or data/evidence collection, wrote review and edited specifically critical review, commentary or revision-including pre- or post-publication stages; S. Kurhan: performed the experiments, or data/evidence collection.Additional informationFundingThis work was supported by Republic of Turkey Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies under a project [TAGEM/16/AR-GE/36].
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
European Journal of Phycology
European Journal of Phycology 生物-海洋与淡水生物学
CiteScore
4.80
自引率
4.20%
发文量
37
审稿时长
>12 weeks
期刊介绍: The European Journal of Phycology is an important focus for the activities of algal researchers all over the world. The Editors-in-Chief are assisted by an international team of Associate Editors who are experts in the following fields: macroalgal ecology, microalgal ecology, physiology and biochemistry, cell biology, molecular biology, macroalgal and microalgal systematics, applied phycology and biotechnology. The European Journal of Phycology publishes papers on all aspects of algae, including cyanobacteria. Articles may be in the form of primary research papers and reviews of topical subjects. The journal publishes high quality research and is well cited, with a consistently good Impact Factor.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信