一种治疗高危角膜移植术后持续性角膜糜烂的新方法

Q4 Medicine
E. V. Chentsova, N. V. Borovkova, D. A. Bozhenko, I. N. Ponomarev, P. V. Makarov, M. V. Storozheva, M. S. Makarov
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引用次数: 0

摘要

目的:比较常规保存羊膜和饱和富血小板血浆裂解液羊膜治疗高危角膜移植术后持续性角膜糜烂(PCE)的疗效。材料和方法。将40例高危穿透性角膜移植术后持续性角膜糜烂患者分为两组,每组20人,随访12个月。主组患者年龄34 ~ 84岁,采用Flexamer羊膜+ PRP裂解液治疗,对照组患者年龄41 ~ 80岁,仅采用Flexamer羊膜治疗。应用羊膜涂层治疗穿透性角膜移植术后保守治疗不成功的持续性角膜糜烂。羊膜在距肢体2 mm处连续缝合,并用软性隐形眼镜覆盖。作为血小板的来源,我们使用健康志愿者的血液,从中提取血小板浓度超过100万个/µl的富血小板血浆,然后在-40°C冷冻,在0…4°C解冻,获得PRP裂解液。评价两组疗效的标准是随访结束时上皮化次数、羊膜涂层数量和保存移植数量。结果。冻干羊膜与自体PRP裂解物生长因子饱和,显示出生物相容性。对患者来说,这种方法是安全的,可以减少上皮化时间,减少用羊膜覆盖PÑE所需的手术次数,并增加透明和半透明穿透性角膜移植物成功植入的可能性。结论。利用富含自体PRP裂解物生长因子的冻干羊膜是治疗高风险角膜移植术后穿透性角膜移植后PCE的一种很有前途的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A new method to treat persistent corneal erosions after high-risk keratoplasty
Purpose : to compare the effectiveness of conventional preserved amnion and amnion saturated with platelet-rich (PRP) plasma lysate for the treatment of persistent corneal erosions (PCE) after high-risk keratoplasty. Materials and methods . 40 patients with persistent corneal erosions after high-risk penetrating keratoplasty, followed up for 12 months, were divided into two clinical groups of 20 people each. The main group of patients, aged 34 to 84, received Flexamer amniotic membrane + PRP lysate, while the comparison group, aged 41 to 80, received Flexamer amniotic membrane only. Amniotic membrane coating was used in persistent corneal erosions of penetrating keratoplasty after unsuccessful conservative treatment. The amnion was sewn 2 mm from the limb with a continuous suture and covered with a soft contact lens. As a source of platelets, we used the blood of healthy volunteers, from which platelet-rich plasma with platelet concentration of over 1000 thousand/µl was taken, which was then frozen at -40 °C and defrosted at 0…4 °C to obtain PRP lysate. The criteria for evaluating the effectiveness of both groups were the times of epithelialization, the number of amniotic membrane coatings, and the number of preserved transplants at the end of the follow-up. Results . Lyophilized amniotic membrane saturated with autologous PRP lysate growth factors was shown to be biocompatible. It was found to be safe for the patients, to reduce the epithelialization time, to reduce the number of operations required to cover the PÑE with the amnion, and to increase the likelihood of successful transparent and translucent engraftment of a penetrating keratograft. Conclusion . The use of a lyophilized amniotic membrane enriched with growth factors of autologous PRP lysate is a promising method for the treatment of PCE of the penetrating corneal graft after high-risk keratoplasty.
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来源期刊
CiteScore
0.50
自引率
0.00%
发文量
107
审稿时长
16 weeks
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