丁香油纳米乳液的优化:抗氧化、抗菌和抗癌性能的评价

IF 2.5 Q3 CHEMISTRY, PHYSICAL
José Nabor Haro-González, Brenda Nathalie Schlienger de Alba, Moisés Martínez-Velázquez, Gustavo Adolfo Castillo-Herrera, Hugo Espinosa-Andrews
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引用次数: 0

摘要

丁香精油传统上被用作麻醉剂、止痛药或杀虫剂,最近,它作为抗微生物、抗氧化剂或抗癌剂的应用已经被探索。纳米乳液是热力学不稳定的分散体(d <100 nm),通过混合两种不混相生产,在许多情况下,它可以提高药物,化妆品或食品应用的功能成分的稳定性和生物活性。本研究采用响应面法对丁香精油纳米乳液的制备工艺进行了优化。通过改变丁香油的百分比(0-100%)、表面活性剂的含量(1-4%)和油相的含量(0-20%),使表面活性剂的浓度降到最低。在最佳条件下,以1.0%的表面活性剂和2.5%的油相(其中丁香精油占50.7%)为原料,制得纳米乳液(93.19±3.92 nm)。在快速稳定性试验(离心、冻融和加热冷却)中,优化后的纳米乳是稳定的,但在不同的温度下,其平均液滴尺寸增大。纳米乳含有的酚含量相当于736毫克没食子酸/毫升。然而,精油的抗氧化能力(IC50 = 0.78µg/mL)在纳米乳中被抵消(IC50 = 2.43µg/mL)。纳米乳的抑菌活性表现出菌株依赖性,MIC范围为0.0468 ~ 0.75 mg/mL,其中大肠杆菌和鼠伤寒沙门氏菌对其最敏感。最后,丁香精油纳米胶囊对Caco-2癌细胞的体外细胞毒活性(227 μg/mL)高于游离丁香精油(283 μg/mL),而丁香精油纳米胶囊(306 μg/mL)在HT-29细胞系的体外细胞毒活性低于精油(231 μg/mL)。这项研究显示丁香精油纳米乳液在开发生物疗法治疗疾病方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimization of Clove Oil Nanoemulsions: Evaluation of Antioxidant, Antimicrobial, and Anticancer Properties
Clove essential oil is traditionally used as an anesthetic, analgesic, or insecticide, and recently, its applications as an antimicrobial, antioxidant, or anticancer agent have been explored. Nanoemulsions are thermodynamically unstable dispersions (d < 100 nm) produced by mixing two immiscible phases, which, in many cases, improve the stability and biological activities of functional ingredients for pharmaceutical, cosmetic, or food applications. This research optimized the formation of clove essential oil nanoemulsions by employing response surface methodology. The surfactant concentration was minimized by modifying the percentage of clove oil (0–100%), surfactant content (1–4%), and oil phase content (0–20%). In the optimum conditions, a nanoemulsion (93.19 ± 3.92 nm) was produced using 1.0% surfactant and 2.5% oil phase of which 50.7% was clove essential oil. The optimized nanoemulsion was stable in rapid stability tests (centrifugation, freezing–thawing, and heating–cooling), but its average droplet size increased during storage at different temperatures. The nanoemulsion contains a phenolic content equivalent to 736 mg gallic acid/mL. However, the antioxidant capacity of the essential oil (IC50 = 0.78 µg/mL) was dismissed in the nanoemulsion (IC50 = 2.43 µg/mL). The antimicrobial activity of the nanoemulsion showed strain–dependent behavior with MIC ranging from 0.0468 to 0.75 mg/mL, where E. coli and S. typhimurium were the most susceptible pathogenic bacteria. Finally, nanoencapsulation of clove oil showed higher in vitro cytotoxic activity against Caco–2 cancer cells (227 μg/mL) than free clove essential oil (283 μg/mL), but nanoemulsion (306 μg/mL) was less effective than oil (231 μg/mL) in the HT–29 line. This research shows the potential of clove essential oil nanoemulsions for developing biological therapies to treat diseases.
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来源期刊
Colloids and Interfaces
Colloids and Interfaces CHEMISTRY, PHYSICAL-
CiteScore
3.90
自引率
4.20%
发文量
64
审稿时长
10 weeks
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