白藜芦醇诱导自噬可增强多沙唑嗪对乳腺癌细胞的抗癌作用。

Q4 Medicine
Ali Sami Ahmed Alhayali, Waseem Ali Hasan, Firas Subhi Salah
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引用次数: 1

摘要

与单一治疗方案相比,联合抗癌药物通过协同或加性方式靶向关键途径提高了疗效。据报道,Doxazosin (DOX)和Resveratrol (RES)对不同的癌细胞具有抗癌作用。目的:评价多沙唑嗪和白藜芦醇单用或联用对乳腺癌细胞增殖的抑制作用。方法:将MCF-7癌细胞接种于混合单层细胞,分别用100、50、25、12.5、6.25、3.12µM的药物单独或联合处理。采用二甲基噻唑基二苯四唑盐(MTT)法和菌落形成法评价细胞毒性。采用免疫细胞化学技术检测caspase-3和GABARAP的表达。结果:所有药物对肿瘤细胞增殖均有显著影响。Doxazosin-Resveratrol联合治疗比单独使用每种药物具有更强的细胞毒性作用,以及MCF-7癌细胞的集落形成。联合治疗时,caspase-3的表达模式高于其他治疗。白藜芦醇处理可提高GABARAP在癌细胞中的表达,表明诱导了自噬过程。结论:虽然每种药物都有其独特的细胞毒性作用,但Doxazosin和白藜芦醇合用可协同抑制MCF-7癌细胞增殖。关键词:白藜芦醇;Doxazosin;结合;MCF-7;自噬。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Autophagy induction using Resveratrol enhances the anti-cancer efficacy of Doxazosin in breast cancer cells.
The combination of anti-cancer drugs improves effectiveness compared to the mono-therapy scenario by targeting key pathways synergistically or in an additive way. Doxazosin (DOX) and Resveratrol (RES) are reported to have an anti-cancer impact against different cancer cells. Aim: To evaluate the anti-cancer properties of Doxazosin and Resveratrol, each alone or in combination, in inhibiting breast cancer cell proliferation. Methods: MCF-7 cancer cells were seeded to a confluent monolayer and treated with 100, 50, 25, 12.5, 6.25, and 3.12 µM of each drug alone and as a combination. Cytotoxicity was evaluated using dimethyl thiazolyl diphenyl tetrazolium salt (MTT) assay and colony formation assay. The immunocytochemistry technique was conducted to evaluate caspase-3 and GABARAP expression. Results: All the drugs have a significant effect on cancer cell proliferation. The combination treatment of Doxazosin-Resveratrol has a more cytotoxic effect than each drug alone, as well as in colony formation of MCF-7 cancer cells. In combination treatment, caspase-3 had a higher expression pattern than other treatments. Resveratrol treatment elevated GABARAP expression in cancer cells, indicating the induction of the autophagy process. Conclusion: Although each drug has its characteristic result of a cytotoxic effect, the combination of Doxazosin and Resveratrol synergizes the inhibition of MCF-7 cancer cell proliferation. Keywords: Resveratrol; Doxazosin; combination; MCF-7; Autophagy.
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来源期刊
Revista Bionatura
Revista Bionatura Medicine-Epidemiology
CiteScore
0.50
自引率
0.00%
发文量
144
审稿时长
8 weeks
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