新戈bius fluviatiis (Pallas, 1814)的染色体带带特性(鲈形目,戈biae)

Sevgi ÜNAL KARAKUŞ, Muradiye KARASU AYATA, Muhammet GAFFAROĞLU
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摘要

首次对分布于台湾的猕猴虾虎鱼Neogobius fluviatilis (Pallas, 1814)进行了细胞遗传学研究。在此背景下,揭示了河蟹的二倍体染色体数目、染色体形态和染色体带带特性(c带和Ag-NOR染色)。采用风干法制备了头肾细胞的染色体载玻片。显微镜下观察染色体载玻片,中期照相。用数字卡尺测量染色体,手工排列核型。二倍体染色体数目为46条。核型由单臂染色体组成。基本臂数也计算为46。核型中未发现异型性染色体。几乎所有染色体的中心粒上都检测到c带。另外,在银染中期发现2个Ag-NORs。本研究利用传统的细胞遗传学技术揭示了基耶氏梭状芽孢杆菌的染色体特性。该报道可能会完善新虾属的细胞遗传学资料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chromosome Banding Properties of Neogobius fluviatilis (Pallas, 1814) (Perciformes, Gobiidae)
The monkey goby, Neogobius fluviatilis (Pallas, 1814) that distributed in Türkiye was studied cytogenetically for the first time. In this context, diploid chromosome number, chromosome morphology and also chromosomal banding properties (C-banding and Ag-NOR staining) of N. fluviatilis were revealed out. Chromosome slides were prepared from head kidney cells according to the air-drying technique. Chromosome slides were observed under the microscope and metaphases were photographed. The chromosomes were measured by digital caliper and karyotype was arranged manually. The diploid chromosome number was found as 46. Karyotype was composed with all uniarmed chromosomes. Fundamental arm number was calculated as 46 too. No heteromorphic sex chromosomes were determined in the karyotype. C-bands were detected on the pericentromeres of almost all chromosomes. Otherwise, two Ag-NORs were found in the silver-stained metaphases. This study revealed out chromosomal properties of N. fluviatilis from Türkiye with conventional cytogenetic techniques. This report may improve the cytogenetic data of the genus Neogobius.
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