柠檬酸对花蕙兰(Cymbidium floribundum)器官发生的影响

Q3 Agricultural and Biological Sciences

Environmental Control in Biology Pub Date : 2023-10-01 DOI:10.2525/ecb.61.69

Anjum Ferdous ONA, Kazuhiko SHIMASAKI

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引用次数: 0

摘要

研究了柠檬酸(CA)对大花蕙兰(Cymbidium floribundum)原球茎样体(PLBs)器官发生的影响，以确定适宜的CA浓度。外植体在添加不同浓度CA的改良MS培养基中培养，在25±1℃、24 h光照条件下培养9周。在改良MS培养基中添加0.1 g/L CA可部分降低培养PLB的褐变强度，显著提高PLB的形成、PLB的新梢形成和新梢的根形成。在改性MS培养基中添加0.1 g/L以上的CA可增加培养plb的褐变强度。综上所述，CA能有效控制花金莲PLB培养中的褐变问题，在改良MS培养基中添加0.1 g/L CA对器官发生的影响最大。

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Effect of Citric Acid on the Organogenesis of <i>Cymbidium floribundum</i>

We examined the effects of citric acid (CA) on organogenesis from protocorm-like bodies (PLBs) of Cymbidium floribundum to determine the appropriate concentration of CA for organogenesis. Explants were cultured in modified MS media supplemented with various concentrations of CA and maintained at 25±1℃ and a 24 h light period for 9 weeks. Addition of 0.1 g/L CA in modified MS media partially reduced the browning intensity in the cultured PLBs, which significantly increased PLB formation, shoot formation from the PLBs, and root formation from the shoots. More than 0.1 g/L CA in modified MS media increased the browning intensity in cultured PLBs. We concluded that CA can effectively control the browning problem in PLB culture of C. floribundum, and addition of 0.1 g/L CA in modified MS media was optimum for organogenesis.

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来源期刊

Environmental Control in Biology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

2.00

自引率

0.00%

发文量