Darko Ašanin, Tina Andrejević, Dušan Milivojević, Nevena Stevanović, Biljana Glišić
{"title":"双核银(I)配合物与酞菁:DNA/BSA结合及体内毒性研究","authors":"Darko Ašanin, Tina Andrejević, Dušan Milivojević, Nevena Stevanović, Biljana Glišić","doi":"10.5937/kgjsci2345079a","DOIUrl":null,"url":null,"abstract":"The present study reports the synthesis and spectroscopic characterization of dinuclear silver(I) complexes, {[Ag(X-O)(phtz-N)]2(µ-phtz-N,N')2} (X = NO3 - (Ag1) and CF3SO3 - (Ag2), and phtz is phthalazine). The interaction of these two complexes with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was investigated to assess their binding affinity to these biomolecules. The binding constants of complexes Ag1 and Ag2 to BSA are found to be higher than those for ct-DNA, being in accordance with their higher affinity toward the studied protein. The values of partition coefficient (logP) for the investigated complexes indicate higher cellular uptake efficiency of Ag1 than Ag2. The in vivo toxicity of the complexes Ag1 and Ag2 against the model organism, Caenorhabditis elegans, was studied to check their therapeutic potential.","PeriodicalId":17829,"journal":{"name":"Kragujevac Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dinuclear silver(I) complexes with phthalazine: DNA/BSA binding and in vivo toxicity study\",\"authors\":\"Darko Ašanin, Tina Andrejević, Dušan Milivojević, Nevena Stevanović, Biljana Glišić\",\"doi\":\"10.5937/kgjsci2345079a\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The present study reports the synthesis and spectroscopic characterization of dinuclear silver(I) complexes, {[Ag(X-O)(phtz-N)]2(µ-phtz-N,N')2} (X = NO3 - (Ag1) and CF3SO3 - (Ag2), and phtz is phthalazine). The interaction of these two complexes with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was investigated to assess their binding affinity to these biomolecules. The binding constants of complexes Ag1 and Ag2 to BSA are found to be higher than those for ct-DNA, being in accordance with their higher affinity toward the studied protein. The values of partition coefficient (logP) for the investigated complexes indicate higher cellular uptake efficiency of Ag1 than Ag2. The in vivo toxicity of the complexes Ag1 and Ag2 against the model organism, Caenorhabditis elegans, was studied to check their therapeutic potential.\",\"PeriodicalId\":17829,\"journal\":{\"name\":\"Kragujevac Journal of Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Kragujevac Journal of Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5937/kgjsci2345079a\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Kragujevac Journal of Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5937/kgjsci2345079a","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Dinuclear silver(I) complexes with phthalazine: DNA/BSA binding and in vivo toxicity study
The present study reports the synthesis and spectroscopic characterization of dinuclear silver(I) complexes, {[Ag(X-O)(phtz-N)]2(µ-phtz-N,N')2} (X = NO3 - (Ag1) and CF3SO3 - (Ag2), and phtz is phthalazine). The interaction of these two complexes with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was investigated to assess their binding affinity to these biomolecules. The binding constants of complexes Ag1 and Ag2 to BSA are found to be higher than those for ct-DNA, being in accordance with their higher affinity toward the studied protein. The values of partition coefficient (logP) for the investigated complexes indicate higher cellular uptake efficiency of Ag1 than Ag2. The in vivo toxicity of the complexes Ag1 and Ag2 against the model organism, Caenorhabditis elegans, was studied to check their therapeutic potential.