高效转基因选择体系的建立及其在甘蔗上的应用

Wenzhi Wang, Jungang Wang, Cuilian Feng, Tingting Zhao, Linbo Shen, Xiaoyan Feng, Shuzhen Zhang
{"title":"高效转基因选择体系的建立及其在甘蔗上的应用","authors":"Wenzhi Wang, Jungang Wang, Cuilian Feng, Tingting Zhao, Linbo Shen, Xiaoyan Feng, Shuzhen Zhang","doi":"10.48130/tp-2023-0011","DOIUrl":null,"url":null,"abstract":"Transgenic strategy plays an important role in the biological study and breeding of sugarcane. However, the efficiency of sugarcane transgenic systems remains disappointing to breeders. Various cultivated varieties are recalcitrant to genetic transformation, and only a few sugarcane research institutes could successfully obtain positive transgenic lines. In our previous research, three kinds of sugarcane transgenic selection systems, namely, the PMI/Mannose, CP4-EPSPS/glyphosate, and <italic>bar</italic>/Basta selection systems, were successfully established. Among these systems, the <italic>bar</italic>/Basta selection system was the most efficient. By applying this selection system, 10 or more transgenic shoots could be obtained from a gram of embryogenic calluses. In addition, the resistant shoots obtained after screening were almost 100% positive for the molecular assay, and all of the transgenic shoots showed high herbicide tolerance in lab tests and field trials. Herein, the key points/steps, advantage and contribution to sugarcane studies and breeding in China of the efficient <italic>bar</italic>/Basta sugarcane transformation system are presented and discussed.","PeriodicalId":120197,"journal":{"name":"Tropical Plants","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Establishment of an efficient transgenic selection system and its utilization in &lt;i&gt;Saccharum officinarum&lt;/i&gt;\",\"authors\":\"Wenzhi Wang, Jungang Wang, Cuilian Feng, Tingting Zhao, Linbo Shen, Xiaoyan Feng, Shuzhen Zhang\",\"doi\":\"10.48130/tp-2023-0011\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Transgenic strategy plays an important role in the biological study and breeding of sugarcane. However, the efficiency of sugarcane transgenic systems remains disappointing to breeders. Various cultivated varieties are recalcitrant to genetic transformation, and only a few sugarcane research institutes could successfully obtain positive transgenic lines. In our previous research, three kinds of sugarcane transgenic selection systems, namely, the PMI/Mannose, CP4-EPSPS/glyphosate, and <italic>bar</italic>/Basta selection systems, were successfully established. Among these systems, the <italic>bar</italic>/Basta selection system was the most efficient. By applying this selection system, 10 or more transgenic shoots could be obtained from a gram of embryogenic calluses. In addition, the resistant shoots obtained after screening were almost 100% positive for the molecular assay, and all of the transgenic shoots showed high herbicide tolerance in lab tests and field trials. Herein, the key points/steps, advantage and contribution to sugarcane studies and breeding in China of the efficient <italic>bar</italic>/Basta sugarcane transformation system are presented and discussed.\",\"PeriodicalId\":120197,\"journal\":{\"name\":\"Tropical Plants\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical Plants\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.48130/tp-2023-0011\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Plants","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.48130/tp-2023-0011","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

转基因策略在甘蔗生物学研究和育种中起着重要的作用。然而,甘蔗转基因系统的效率仍然让育种者感到失望。许多栽培品种对遗传转化具有抗性,只有少数甘蔗研究机构能够成功获得阳性转基因品系。在我们前期的研究中,成功建立了PMI/甘露糖、CP4-EPSPS/草甘膦和bar/Basta三种甘蔗转基因选择体系。在这些系统中,bar/Basta选择系统是最有效的。应用该选择系统,每克胚性愈伤组织可获得10个以上的转基因芽。此外,筛选后获得的抗性芽在分子检测中几乎100%呈阳性,所有转基因芽在实验室试验和田间试验中都表现出较高的耐除草剂性。本文介绍了bar/Basta甘蔗高效转化体系的要点/步骤、优势及其对中国甘蔗研究和育种的贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Establishment of an efficient transgenic selection system and its utilization in <i>Saccharum officinarum</i>
Transgenic strategy plays an important role in the biological study and breeding of sugarcane. However, the efficiency of sugarcane transgenic systems remains disappointing to breeders. Various cultivated varieties are recalcitrant to genetic transformation, and only a few sugarcane research institutes could successfully obtain positive transgenic lines. In our previous research, three kinds of sugarcane transgenic selection systems, namely, the PMI/Mannose, CP4-EPSPS/glyphosate, and bar/Basta selection systems, were successfully established. Among these systems, the bar/Basta selection system was the most efficient. By applying this selection system, 10 or more transgenic shoots could be obtained from a gram of embryogenic calluses. In addition, the resistant shoots obtained after screening were almost 100% positive for the molecular assay, and all of the transgenic shoots showed high herbicide tolerance in lab tests and field trials. Herein, the key points/steps, advantage and contribution to sugarcane studies and breeding in China of the efficient bar/Basta sugarcane transformation system are presented and discussed.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信