None R. N. B. Athalla, None C. D. Nugraha, None Suyadi
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引用次数: 0
摘要
人工授精(AI)是一种旨在提高牲畜生产力,从而使动物具有优良遗传的生殖技术。在低温下储存精液会损害精子质量,提高精液质量的一种方法是添加抗氧化剂,如槲皮素,这已被证明对精子质量有积极影响。本研究的目的是研究槲皮素对5岁波尔山羊人工阴道冷藏过程中精子质量的影响。本研究采用完全随机设计(CRD)因子双因素法。检测的变量是精子的活力、活力、异常和膜完整性。采用方差分析(ANOVA)对数据进行分析,如果存在显著或显著的治疗差异,则继续采用Duncan 's Multiple Range Test (DMRT)。结果表明,槲皮素添加量和贮藏时间对细胞的运动性、活力和膜完整性的影响有显著差异(p < 0.01),对异常率的影响无显著差异(p < 0.05)。结果表明,槲皮素经Andromed扩展剂稀释30 μM,冷藏24小时处理效果最好。
Effect of Concentration of Antioxidant Quercetin on Semen Quality of Boer Goat during Cold Storage using Andromed Extender
Artificial insemination (AI) is a reproductive technology aimed at increasing livestock productivity resulting in animals with superior genetics. Storing semen at cold temperatures can damage sperm quality, one way to improve semen quality is by adding antioxidants such as Quercetin, which has been shown to have a positive effect on sperm quality. The objective of this research is to determine the effect of Quercetin on sperm quality in cold storage using Andromed extender on 5 years old Boer goat using artificial vagina. The method of this research was using Completed Randomized Design (CRD) Factoral two-factors. Variable being tested was motility, viability, abnormality and membrane integrity of spermatozoa. Data was analyze using Analysis of Variance (ANOVA), if there was a significant or significantly differences of treatment then continued by Duncan’s Multiple Range Test (DMRT). Result showed that additional of quercetin and storage time had a significantly differences (P<0.01) effect on motility, viability and membrane integrity and did not have significant (P<0.05) effect on percentage of abnormality. The best result shown that additional of quercetin at 30 μM diluted with Andromed extender for 24-hour cold storage has the best treatment for semen quality.