Jungnam Kim, Hyunwoo Choi, Seoin Yang, Insung S. Choi
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Promoting effects of cannabidiol on neurite growth and neuronal development in neuron-astrocyte sandwich coculture
(–)-Cannabidiol (CBD), a nonpsychoactive compound isolated from the Cannabis genus, has recently emerged as a promising research subject in neuroscience due to its potential therapeutic benefits against neurological disorders. However, there has been limited fundamental understanding of how CBD affects neuronal development, such as neurite outgrowth and axonal branching, at the cell level. This work investigates the effects of CBD on the early development of primary hippocampal neurons in a noncontact neuron-astrocyte coculture system. The immunocytochemical and quantitative analyses indicate that CBD of 10 μM promotes neurite growth, including neurite elongation and arborization, and accelerates the process of axon specification. The CBD treatment to the coculture system leads to noticeable increases in the longest-neurite length, primary-neurite number, and branch-point number, as well as the population of axon-determined neurons. The results provide informative insights into the therapeutic potential of CBD in neurological disorders, such as Alzheimer's disease.
期刊介绍:
The Bulletin of the Korean Chemical Society is an official research journal of the Korean Chemical Society. It was founded in 1980 and reaches out to the chemical community worldwide. It is strictly peer-reviewed and welcomes Accounts, Communications, Articles, and Notes written in English. The scope of the journal covers all major areas of chemistry: analytical chemistry, electrochemistry, industrial chemistry, inorganic chemistry, life-science chemistry, macromolecular chemistry, organic synthesis, non-synthetic organic chemistry, physical chemistry, and materials chemistry.