鲨鱼软骨提取物中硫酸软骨素的微观结构及其应用前景

Q3 Environmental Science
Titiek Indhira Agustin, Risma Risma, Retno Sari, Dwi Setyawan
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引用次数: 0

摘要

鲨鱼(Prionace glauca)软骨是Sidoarjo市冷冻鱼行业的工业废物。以前的研究表明,软骨含有生物活性化合物、葡萄糖胺和软骨素,但提取的产物不稳定(在储存过程中容易融化),而且有氨气味。本研究的目的是通过添加不同的吸收剂来获得稳定的无氨气味的提取物产品。实验采用完全随机设计,应用吸收剂、HPMC、Cab-Osil和Avicel PH 101。测定了鲨鱼提取物产品的产率和感官性能。显微结构观察采用扫描电镜(SEM),硫酸软骨素含量分析采用高效液相色谱(HPLC)。结果表明,添加不同的吸收剂对鲨鱼软骨提取物的收率和感官有显著影响。Avicel PH 101(90%)、Cab-Osil(9%)和羟丙基甲基纤维素(HPMC)(1%)的提取量最高,达5.02 g(16.73%)。感受器无嗅盐,呈白米色,结构干燥(稳定)。在这种处理下,鲨鱼软骨提取物产品的微观结构具有致密的表面柔软的结构。相比之下,没有添加吸收剂的提取物具有尖锐的针状微观结构。在此处理下,鲨鱼软骨提取物产品的硫酸软骨素含量最高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Microstructure and Potential of Chondroitin Sulfate in Shark Cartilage Extract
Shark (Prionace glauca) cartilage is an industrial waste of the frozen fish industry in Sidoarjo city. Previous studies indicate that the cartilage contains bioactive compounds, glucosamine, and chondroitin, but the extracted product is unstable (easily melted during storage) and smelly ammonia. This study aims to obtain a stable extract product without the smell of ammonia by adding different absorbers. The experiment used a Complete Randomized Design on applying absorbers, HPMC, Cab-Osil, and Avicel PH 101. The parameters measured were the yield and the organoleptic of the shark’s extract product. The microstructural observations used a Scanning Electron Microscope (SEM), while the chondroitin sulfate content analyses used high-performance liquid chromatography(HPLC). Results showed that adding different absorbers significantly influenced the yield and organoleptic of the shark’s cartilage extract. The use of Avicel PH 101 (90%), Cab-Osil (9%), and Hydroxy Propyl Methyl Cellulose (HPMC) (1%) gave the highest amount of extract, as much as 5.02 g (16.73%). The organoleptic was without smelling salts, whitish beige color, and dry structure (stable). The microstructure of the shark’s cartilage extract product at this treatment had a dense structure with a soft surface. In contrast, the extract without an absorber addition had a sharp, needle-like microstructure. The chondroitin sulfate content of the shark’s cartilage extract product was the highest at this treatment.
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
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