{"title":"1,2,3,4,6-五- o -没食子酰-β- d -葡萄糖对C2C12细胞分化及地塞米松诱导的肌萎缩的影响","authors":"Sung-Hoon Hur, Seong-Uk Lee","doi":"10.35159/kjss.2023.10.32.5.813","DOIUrl":null,"url":null,"abstract":"PURPOSE: In this study, we investigated the effect of 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG) on cell differentiation and dexamethasone-induced muscle atrophy in mouse C2C12 myoblast.BR METHOD: C2C12 myoblasts were cultured with Dulbecco’s modified Eagle’s medium (DMEM), 10% fetal bovine serum (FBS) and were differentiated to myotube cells using a differentiation medium containing 2% horse serum for 4 days. A muscle atrophy model was generated by treating with 1 μM dexamathasone for 18 h. We determined the cell viability, microscopy, western blot, and quantitative real-time PCR analysis.BR RESULTS: PGG had neither effect on cell viability of C2C12 myoblast at a concentration of 0-10 μM for 24 h nor protein and mRNA expression (Atrogin, Murf1, Myogenin, and MyHC).BR CONCLUSIONS: These data indicate that PGG has no effect on cell differentiation and dexamethasoneinduced atrophy using C2C12 cells. Further studies are required to determine the interaction of nutrition and exercise on skeletal muscle atrophy.","PeriodicalId":497986,"journal":{"name":"The Korean Society of Sports Science","volume":"496 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The effect of 1,2,3,4,6-penta-O-galloyl-β-D-gluocse on cell differentiation and dexamethasone-induced muscle atrophy in C2C12 cell\",\"authors\":\"Sung-Hoon Hur, Seong-Uk Lee\",\"doi\":\"10.35159/kjss.2023.10.32.5.813\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"PURPOSE: In this study, we investigated the effect of 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG) on cell differentiation and dexamethasone-induced muscle atrophy in mouse C2C12 myoblast.BR METHOD: C2C12 myoblasts were cultured with Dulbecco’s modified Eagle’s medium (DMEM), 10% fetal bovine serum (FBS) and were differentiated to myotube cells using a differentiation medium containing 2% horse serum for 4 days. A muscle atrophy model was generated by treating with 1 μM dexamathasone for 18 h. We determined the cell viability, microscopy, western blot, and quantitative real-time PCR analysis.BR RESULTS: PGG had neither effect on cell viability of C2C12 myoblast at a concentration of 0-10 μM for 24 h nor protein and mRNA expression (Atrogin, Murf1, Myogenin, and MyHC).BR CONCLUSIONS: These data indicate that PGG has no effect on cell differentiation and dexamethasoneinduced atrophy using C2C12 cells. Further studies are required to determine the interaction of nutrition and exercise on skeletal muscle atrophy.\",\"PeriodicalId\":497986,\"journal\":{\"name\":\"The Korean Society of Sports Science\",\"volume\":\"496 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-10-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Korean Society of Sports Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.35159/kjss.2023.10.32.5.813\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Korean Society of Sports Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35159/kjss.2023.10.32.5.813","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
目的:研究1,2,3,4,6-五- o -没食子酰-β- d -葡萄糖(PGG)对小鼠C2C12成肌细胞分化和地塞米松诱导的肌萎缩的影响。BR方法:将C2C12成肌细胞用Dulbecco 's modified Eagle 's medium (DMEM)和10%胎牛血清(FBS)培养，用含2%马血清的分化培养基培养4 d后分化为肌管细胞。1 μM地塞米松作用18 h，形成肌肉萎缩模型。测定细胞活力、显微镜、western blot和实时荧光定量PCR分析。结果:PGG对C2C12成肌细胞0 ~ 10 μM浓度作用24 h后细胞活力无影响，对Atrogin、Murf1、Myogenin、MyHC蛋白和mRNA表达无影响。结论:这些数据表明PGG对C2C12细胞分化和地塞米松诱导的细胞萎缩无影响。需要进一步的研究来确定营养和运动对骨骼肌萎缩的相互作用。
The effect of 1,2,3,4,6-penta-O-galloyl-β-D-gluocse on cell differentiation and dexamethasone-induced muscle atrophy in C2C12 cell
PURPOSE: In this study, we investigated the effect of 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG) on cell differentiation and dexamethasone-induced muscle atrophy in mouse C2C12 myoblast.BR METHOD: C2C12 myoblasts were cultured with Dulbecco’s modified Eagle’s medium (DMEM), 10% fetal bovine serum (FBS) and were differentiated to myotube cells using a differentiation medium containing 2% horse serum for 4 days. A muscle atrophy model was generated by treating with 1 μM dexamathasone for 18 h. We determined the cell viability, microscopy, western blot, and quantitative real-time PCR analysis.BR RESULTS: PGG had neither effect on cell viability of C2C12 myoblast at a concentration of 0-10 μM for 24 h nor protein and mRNA expression (Atrogin, Murf1, Myogenin, and MyHC).BR CONCLUSIONS: These data indicate that PGG has no effect on cell differentiation and dexamethasoneinduced atrophy using C2C12 cells. Further studies are required to determine the interaction of nutrition and exercise on skeletal muscle atrophy.
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