不同品种牛肌肉卫星细胞特性对高效生产培养肉的影响

IF 2.7 3区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Animal Science and Technology Pub Date : 2023-10-18 DOI:10.5187/jast.2023.e115

Yun-a Kim, Sehyuk Oh, Gyutae Park, Sanghun Park, Yunhwan Park, Hyunsoo Choi, Minjung Kim, Jungseok Choi

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引用次数: 0

摘要

本研究的目的是比较三种供体卫星细胞用于培养肉的体外性能，选择最佳供体，为培养肉生产的供体选择提供参考。从韩宇牛、荷斯坦牛和济州黑牛的肌肉组织中分离牛肌肉卫星细胞，用流式细胞仪进行分选。关于卫星细胞的增殖，所有三个品种都表现出相似的趋势。从PAX7和MYOD mRNA表达水平来看，汉犬和荷斯坦犬的成肌电位较高。通过免疫荧光染色计算肌球蛋白的面积、宽度和融合指数，证实在Hanwoo和Holstein中肌球蛋白呈向上表达。此外，经证实，霍尔斯坦

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Characteristics of Bovine Muscle Satellite Cell from Different Breeds for Efficient Production of Cultured Meat

The purpose of this study was comparing in vitro performances of three breeds of donor satellite cells for cultured meat and selecting the optimal donor and providing insight into the selection of donors for cultured meat production. Cattle muscle satellite cells were isolated from the muscle tissue of Hanwoo, Holstein, and Jeju black cattle, and then sorted by FACS. Regarding proliferation of satellite cells, all three breeds showed similar trends. The myogenic potential was higher for Hanwoo and Holstein breeds based on PAX7 and MYOD mRNA expression levels. When the area, width, and fusion index of the myotube were calculated through immunofluorescence staining of myosin, it was confirmed that it was expressed upward in Hanwoo and Holstein. In addition, it was confirmed that Holstein

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来源期刊

Journal of Animal Science and Technology Agricultural and Biological Sciences-Food Science

CiteScore

4.50

自引率

8.70%

发文量

审稿时长

7 weeks

期刊介绍： Journal of Animal Science and Technology (J. Anim. Sci. Technol. or JAST) is a peer-reviewed, open access journal publishing original research, review articles and notes in all fields of animal science. Topics covered by the journal include: genetics and breeding, physiology, nutrition of monogastric animals, nutrition of ruminants, animal products (milk, meat, eggs and their by-products) and their processing, grasslands and roughages, livestock environment, animal biotechnology, animal behavior and welfare. Articles generally report research involving beef cattle, dairy cattle, pigs, companion animals, goats, horses, and sheep. However, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will also be considered for publication. The Journal of Animal Science and Technology (J. Anim. Technol. or JAST) has been the official journal of The Korean Society of Animal Science and Technology (KSAST) since 2000, formerly known as The Korean Journal of Animal Sciences (launched in 1956).