先天性胸壁和脊柱畸形的绵羊模型:从出生到 3 个月的随访

IF 3.4 3区 医学 Q1 ORTHOPEDICS
JOR Spine Pub Date : 2023-10-18 DOI:10.1002/jsp2.1295
Jesse Shen, Nathalie Samson, Jérôme Lamontagne-Proulx, Denis Soulet, Yves Tremblay, Marc Bazin, Charlène Nadeau, Sarah Bouchard, Jean-Paul Praud, Stefan Parent
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引用次数: 0

摘要

背景 对先天性脊柱和胸壁畸形(CWD)引起的肺部改变的演变和治疗方法知之甚少。大多数产后建立的 CWD 动物模型都没有进行呼吸功能评估。我们的研究旨在评估子宫内诱导的 CWD 对绵羊模型肺生长和功能的影响。 方法 在妊娠 70-75 天时,通过切除左侧第 7 和第 8 根肋骨,对 14 个雌性胎儿进行子宫内 CWD 诱导。每个未做手术的双胎作为对照。产后第一周以及 1、2 和 3 个月时对呼吸力学进行了研究。在 3 个月时还对死后呼吸力学和肺组织形态学进行了评估。 结果 14只CWD羔羊中有8只(57%)和14只对照组羔羊在出生后存活。诱发了1例严重畸形和5例轻度畸形。出生时,CWD羔羊的吸气量(25 mL/kg对对照组的32 mL/kg)、动态(1.4 mL/cmH2O/kg对1.8 mL/cmH2O/kg)和静态(2.0 mL/cmH2O/kg对2.5 mL/cmH2O/kg)呼吸系统顺应性均下降。除了出生 1 个月时吸气能力略有下降外,此后在体内测量的呼吸力学中未观察到其他差异。死后测量发现,CWD羔羊的肺顺应性显著下降,包括每个肺和两个肺的顺应性。与对照组相比,3 个月大的 CWD 动物的肺组织学未发现任何差异。 结论 我们的研究首次评估了产前诱导的 CWD 对绵羊模型从出生到 3 个月肺发育和功能的影响。我们的研究结果表明,与对照组相比,CWD羔羊在3个月时的肺组织形态学没有明显差异。出生时出现的呼吸力学改变在1个月时得到缓解,这可能与诱发严重畸形的挑战有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Ovine model of congenital chest wall and spine deformity: From birth to 3 months follow-up

Ovine model of congenital chest wall and spine deformity: From birth to 3 months follow-up

Background

The evolution and treatment of lung alterations related to congenital spine and chest wall deformities (CWD) are poorly understood. Most animal models of CWD created postnatally were not evaluated for respiratory function. The goal of our study was to evaluate the effects of a CWD induced in utero on lung growth and function in an ovine model.

Methods

A CWD was induced in utero at 70–75 days of gestation in 14 ovine fetuses by resection of the 7th and 8th left ribs. Each non-operated twin fetus was taken as control. Respiratory mechanics was studied postnatally in the first week and at 1, 2, and 3 months. Post-mortem respiratory mechanics and lung histomorphometry were also assessed at 3 months.

Results

Eight out of 14 CWD lambs (57%) and 14 control lambs survived the postnatal period. One severe and five mild deformities were induced. At birth, inspiratory capacity (25 vs. 32 mL/kg in controls), and dynamic (1.4 vs. 1.8 mL/cmH2O/kg), and static (2.0 vs. 2.5 mL/cmH2O/kg) respiratory system compliances were decreased in CWD lambs. Apart from a slight decrease in inspiratory capacity at 1 month of life, no other differences were observed in respiratory mechanics measured in vivo thereafter. Postmortem measurements found a significant decrease in lung compliance—for each lung and for both lungs taken together—in CWD lambs. No differences in lung histology were detected at 3 months in CWD animals compared to controls.

Conclusions

Our study is the first to assess the effects of a prenatally induced CWD on lung development and function from birth to 3 months in an ovine model. Our results show no significant differences in lung histomorphometry at 3 months in CWD lambs compared to controls. Resolution at 1 month of the alterations in respiratory mechanics present at birth may be related to the challenge in inducing severe deformities.

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来源期刊
JOR Spine
JOR Spine ORTHOPEDICS-
CiteScore
6.40
自引率
18.90%
发文量
42
审稿时长
10 weeks
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