Phytochemical Profile And Toxicity Testing Of African Leaf Extract (Vernonia Amygdalina Delile) With Liquid Variation Scavenger Method Brine Shrimp Lethality Test (BSLT)

Background: The African leaf (Vernonia amygdalina Delile) is one of the plants used as a traditional medicine, one of which is anti-cancer. Aim: The study aims to identify compound groups, toxic effects and LC50 values of African leaf extracts with variations in the leaf fluid. Method: In this study, African leaves were extracted using a maseration method with three fluids namely n-hexane, ethyl acetate and 70% ethanol. The extract was qualitatively studied by the group of chemical compounds and toxicity tests were conducted using the method of Brine Shrimp Lethality Test. (BSLT). Result: The results of this study showed that n-hexane extract contains flavonoid and glycoside compounds. Ethyl acetate extract has a flavonoid compound, saponins and glucosides. The results of a linear regression analysis showed that 70% ethanol extract was an extract with the highest LC50value with a LC50 value of 31.80 ppm, an ethyl acetate extracts with LC50 values of 51.70 ppm and n-hexane extracts with LC50, with a 91.88 ppm value of LC50. Conclusion: This study showed that the three extracts included a strong toxic ketegori with a range of 0-100 ppm.