具有杀虫特性的球形赖氨酸芽孢杆菌菌株1795基因组序列数据草图

IF 2.2 Q3 COMPUTER SCIENCE, INFORMATION SYSTEMS
Data Pub Date : 2023-11-03 DOI:10.3390/data8110167
Maria N. Romanenko, Maksim A. Nesterenko, Anton E. Shikov, Anton A. Nizhnikov, Kirill S. Antonets
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引用次数: 0

摘要

球形赖氨酸芽孢杆菌具有重要的农业意义,能够产生杀虫毒素和不同的抗菌和杀真菌活性的化学成分。本文报道了球形L. sphaericus菌株1795的基因组。利用HiSeq X平台上测序的Illumina短读序列,应用SPAdes v3.15.4软件获得基因组的组装。基于23个contigs的累积长度,基因组大小达到4.74 Mb, N50分别为1.34 Mb。组装的基因组携带4672个基因,其中编码蛋白的基因4643个,其中5个位点编码对双翅目、鳞翅目和bltodea有活性的杀虫毒素。我们还发现了负责合成具有抗菌、杀真菌和促进生长特性的次生代谢物的生物合成基因簇。提供的基因组数据将有助于加深我们对遗传标记的理解,这些遗传标记决定了球形L.菌株1795的有效应用,主要用于兽医和医疗中几种吸血昆虫的生物防治目的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Draft Genome Sequence Data of Lysinibacillus sphaericus Strain 1795 with Insecticidal Properties
Lysinibacillus sphaericus holds a significant agricultural importance by being able to produce insecticidal toxins and chemical moieties of varying antibacterial and fungicidal activities. In this study, the genome of the L. sphaericus strain 1795 is presented. Illumina short reads sequenced on the HiSeq X platform were used to obtain the genome’s assembly by applying the SPAdes v3.15.4 software. The genome size based on a cumulative length of 23 contigs reached 4.74 Mb, with a respective N50 of 1.34 Mb. The assembled genome carried 4672 genes, including 4643 protein-encoding ones, 5 of which represented loci coding for insecticidal toxins active against the orders Diptera, Lepidoptera, and Blattodea. We also revealed biosynthetic gene clusters responsible for the synthesis of secondary metabolites with predicted antibacterial, fungicidal, and growth-promoting properties. The genomic data provided will be helpful for deepening our understanding of genetic markers determining the efficient application of the L. sphaericus strain 1795 primarily for biocontrol purposes in veterinary and medical applications against several groups of blood-sucking insects.
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来源期刊
Data
Data Decision Sciences-Information Systems and Management
CiteScore
4.30
自引率
3.80%
发文量
0
审稿时长
10 weeks
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