上调Sirtuin 6表达对非小细胞肺癌A549细胞生物学特性的影响

IF 0.4 4区 医学 Q4 PHARMACOLOGY & PHARMACY
Bojin Zhu, Zheng Wu
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引用次数: 0

摘要

本研究的目的是研究sirtuin 6过表达对A549细胞生物学特性的影响,A549细胞是一种非小细胞肺癌。在实验室环境下,通过引入过表达质粒(质粒克隆脱氧核糖核酸3.1-sirtuin 6),实现了sirtuin 6在A549细胞中的表达上调。随后,通过逆转录-聚合酶链反应和Western blot分析,评估转染A549细胞中sirtuin 6的表达。使用流式细胞术评估增殖,使用Transwell培养系统检测转移和侵袭潜力。此外,利用末端脱氧核苷酸转移酶dUTP缺口末端标记法测定细胞凋亡。结果显示,与对照组和质粒克隆脱氧核糖核酸3.1组相比,质粒克隆脱氧核糖核酸3.1-sirtuin 6组sirtuin 6的表达量显著升高(p<0.05)。此外,转染A549细胞可抑制细胞增殖和侵袭能力,对细胞凋亡无明显影响。综上所述,这些发现表明sirtuin 6的过度表达会阻碍A549细胞的生长和侵袭能力。靶向sirtuin 6可能是治疗非小细胞肺癌的一种有前景的治疗策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Up-Regulation of Sirtuin 6 Expression on the Biological Characteristics of Non-Small Cell Lung Cancer A549 cells
The purpose of this research was to examine the effects of sirtuin 6 overexpression on the biological characteristics of A549 cells, which are a form of non-small cell lung cancer. In the laboratory setting, the upregulation of sirtuin 6 expression in A549 cells was accomplished by introducing an overexpression plasmid (plasmid cloning deoxyribonucleic acid 3.1-sirtuin 6). Subsequently, the expression of sirtuin 6 in the transfected A549 cells was assessed through both reverse transcription-polymerase chain reaction and Western blot analysis. Proliferation was evaluated using flow cytometry, while metastatic and invasive potential were examined using the Transwell cultivation system. Additionally, apoptosis was measured utilizing the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The results revealed a significant increase (p<0.05) in sirtuin 6 expression in the plasmid cloning deoxyribonucleic acid 3.1-sirtuin 6 group compared to the control and plasmid cloning deoxyribonucleic acid 3.1 groups. Moreover, the transfection of A549 cells resulted in suppressed proliferation and invasive ability, without any noticeable impact on cell apoptosis. In conclusion, these findings demonstrate that excessive sirtuin 6 expression impedes the growth and invasive capacity of A549 cells. Targeting sirtuin 6 may represent a promising therapeutic strategy for non-small cell lung cancer treatment.
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来源期刊
自引率
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审稿时长
2 months
期刊介绍: The Indian Journal of Pharmaceutical Sciences (IJPS) is a bi-monthly Journal, which publishes original research work that contributes significantly to further the scientific knowledge in Pharmaceutical Sciences (Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy and Phytochemistry, Pharmacology and Therapeutics, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Pharmacovigilance, Pharmacoepidemiology, Pharmacoeconomics, Drug Information, Patient Counselling, Adverse Drug Reactions Monitoring, Medication Errors, Medication Optimization, Medication Therapy Management, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest). The Journal publishes original research work either as a Full Research Paper or as a Short Communication. Review Articles on current topics in Pharmaceutical Sciences are also considered for publication by the Journal.
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