喜马偕尔邦温泉产脂肪酶耐热枯草芽孢杆菌TTP-06的分离

IF 0.2 Q4 Biochemistry, Genetics and Molecular Biology

Research Journal of Biotechnology Pub Date : 2023-10-15 DOI:10.25303/1811rjbt0960105

Manpreet Kaur, Divya Sharma, Reena Gupta

{"title":"喜马偕尔邦温泉产脂肪酶耐热枯草芽孢杆菌TTP-06的分离","authors":"Manpreet Kaur, Divya Sharma, Reena Gupta","doi":"10.25303/1811rjbt0960105","DOIUrl":null,"url":null,"abstract":"Out of fifty-three isolates isolated from soil samples of Tattapani hot spring of Himachal Pradesh, India, nineteen were screened for lipase production. Isolate TTP-06 with lipase activity of 3.01±0.05 U/ml was characterized and optimized for different production conditions to give maximum production of lipase. Isolate TTP-06 was identified as Bacillus subtilis TTP-06. Maximum enzyme activity was observed when 0.75% (v/v) of 24 hrs old inoculum was used in production medium (pH 8.0) containing 2.0% (v/v) olive oil, 0.75% (w/v) peptone, 0.75% (w/v) glucose, 0.3% (w/v) NaCl, 0.05% (w/v) MgSO4 and incubated at 55ºC under shaking conditions (150 rpm) for 24 hrs.","PeriodicalId":21091,"journal":{"name":"Research Journal of Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.2000,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation of lipase producing thermotolerant Bacillus subtilis TTP-06 from hot spring of Himachal Pradesh\",\"authors\":\"Manpreet Kaur, Divya Sharma, Reena Gupta\",\"doi\":\"10.25303/1811rjbt0960105\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Out of fifty-three isolates isolated from soil samples of Tattapani hot spring of Himachal Pradesh, India, nineteen were screened for lipase production. Isolate TTP-06 with lipase activity of 3.01±0.05 U/ml was characterized and optimized for different production conditions to give maximum production of lipase. Isolate TTP-06 was identified as Bacillus subtilis TTP-06. Maximum enzyme activity was observed when 0.75% (v/v) of 24 hrs old inoculum was used in production medium (pH 8.0) containing 2.0% (v/v) olive oil, 0.75% (w/v) peptone, 0.75% (w/v) glucose, 0.3% (w/v) NaCl, 0.05% (w/v) MgSO4 and incubated at 55ºC under shaking conditions (150 rpm) for 24 hrs.\",\"PeriodicalId\":21091,\"journal\":{\"name\":\"Research Journal of Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2023-10-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Research Journal of Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.25303/1811rjbt0960105\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research Journal of Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.25303/1811rjbt0960105","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 0

摘要

从印度喜马偕尔邦塔塔帕尼温泉土壤样品中分离的53株分离菌中，有19株产脂肪酶。对脂肪酶活性为3.01±0.05 U/ml的分离菌株TTP-06进行了表征，并对不同的生产条件进行了优化，以获得最大的脂肪酶产量。分离物TTP-06经鉴定为枯草芽孢杆菌TTP-06。将0.75% (v/v)的24小时接种物置于含有2.0% (v/v)橄榄油、0.75% (w/v)蛋白胨、0.75% (w/v)葡萄糖、0.3% (w/v) NaCl、0.05% (w/v) MgSO4的生产培养基(pH 8.0)中，55℃振荡条件下(150 rpm)孵育24小时，酶活性最高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Isolation of lipase producing thermotolerant Bacillus subtilis TTP-06 from hot spring of Himachal Pradesh

Out of fifty-three isolates isolated from soil samples of Tattapani hot spring of Himachal Pradesh, India, nineteen were screened for lipase production. Isolate TTP-06 with lipase activity of 3.01±0.05 U/ml was characterized and optimized for different production conditions to give maximum production of lipase. Isolate TTP-06 was identified as Bacillus subtilis TTP-06. Maximum enzyme activity was observed when 0.75% (v/v) of 24 hrs old inoculum was used in production medium (pH 8.0) containing 2.0% (v/v) olive oil, 0.75% (w/v) peptone, 0.75% (w/v) glucose, 0.3% (w/v) NaCl, 0.05% (w/v) MgSO4 and incubated at 55ºC under shaking conditions (150 rpm) for 24 hrs.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Research Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

0.60

自引率

0.00%

发文量

192

审稿时长

1.5 months

期刊介绍： We invite you to contribute Research Papers / Short Communications / Review Papers: -In any field of Biotechnology, Biochemistry, Microbiology and Industrial Microbiology, Soil Technology, Agriculture Biotechnology. -in any field related to Food Biotechnology, Nutrition Biotechnology, Genetic Engineering and Commercial Biotechnology. -in any field of Biotechnology related to Drugs and Pharmaceutical products for human beings, animals and plants. -in any field related to Environmental Biotechnolgy, Waste Treatment of Liquids, Soilds and Gases; Sustainability. -in inter-realted field of Chemical Sciences, Biological Sciences, Environmental Sciences and Life Sciences. -in any field related to Biotechnological Engineering, Industrial Biotechnology and Instrumentation. -in any field related to Nano-technology. -in any field related to Plant Biotechnology.