日惹地区大毒蛇内生真菌的细胞毒性筛选

IF 0.7 Q4 PHARMACOLOGY & PHARMACY
Puji Astuti, Ikra Agustina, Caterina Siskadewi Lay, Triana Hertiani
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引用次数: 0

摘要

癌症是一个全球性问题,发病率和死亡率都很高。印度尼西亚拥有生物多样性,作为药用原料来源具有巨大潜力。已知含有活性代谢物,具有潜在的抗癌作用。据报道,一些内生植物能够产生与其亲本植物相似的次生代谢物。本研究旨在获得具有抗癌潜力的内生真菌。通过筛选从日惹地区收集的巨叶藻(P. macrocarpa)部分产生次生代谢物的内生真菌,探索产生抗癌化合物的内生真菌。使用癌细胞T47D、MCF7、HeLa和正常Vero细胞进行体外抗癌生物测定(MTT)。利用流式细胞术追踪潜在提取物的细胞周期调节和凋亡机制。首先,对产生潜在细胞毒性提取物的真菌进行了真菌鉴定。采用LC-HRMS对其含量进行分析。结果表明,叶片内生提取物(代码PC-L2)对MCF-7细胞的杀伤效果最好,IC50值为110.66µg/mL。产PC-L2提取物的真菌与文平Clonostachys wenpingii菌株DUCC5606密切相关。该提取物诱导细胞凋亡,但不是通过细胞周期调节机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytotoxicity Screening of Endophytic Fungi from Phaleria macrocarpa (Scheff) Boerl) Collected in Yogyakarta District
Cancer is a disease that is a global problem with a high prevalence and mortality rate. Indonesia has biodiversity with enormous potential as a source of medicinal ingredients. Phaleria macrocarpa (Scheff) Boerl) It is known to contain active metabolites, potentially an anticancer. Some endophytes are reported to be able to produce secondary metabolites similar to their parent plants. This study aimed to obtain endophytic fungi from P. macrocarpa capable of producing compounds with anticancer potential. Endophytes producing anticancer compounds were explored by screening endophytic fungi producing secondary metabolites from parts of the plant P. macrocarpa collected in the Yogyakarta area. The anticancer bioassay (MTT assay) was performed in vitro using cancer cells T47D, MCF7, HeLa, and normal Vero cells. Potential extracts were traced for their mechanism of cell cycle modulation and apoptosis using Flowcytometry. First, fungal identification was carried out on fungi producing potential cytotoxic extracts. Then the compound content was analyzed using LC-HRMS. The results from the six extracts obtained from fruit, leaf, and stem parts showed that the endophytic extract from the leaves (code PC-L2) had the best cytotoxicity compared to other endophytes and showed the best result on MCF-7 cells with an IC50 value of 110.66 µg/mL. The producing PC-L2 extract fungi are closely associated with Clonostachys wenpingii strain DUCC5606. This extract induced apoptosis but not through the mechanism of cell cycle modulation.
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来源期刊
INDONESIAN JOURNAL OF PHARMACY
INDONESIAN JOURNAL OF PHARMACY PHARMACOLOGY & PHARMACY-
CiteScore
1.20
自引率
0.00%
发文量
38
审稿时长
12 weeks
期刊介绍: The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education. The journal includes various fields of pharmaceuticals sciences such as: -Pharmacology and Toxicology -Pharmacokinetics -Community and Clinical Pharmacy -Pharmaceutical Chemistry -Pharmaceutical Biology -Pharmaceutics -Pharmaceutical Technology -Biopharmaceutics -Pharmaceutical Microbiology and Biotechnology -Alternative medicines.
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