Extraction of starch and phenolic compounds from Mangifera indica L. var. Kesar seeds and its characterization

The present study is aimed to examine the effect of various parameters (pH, solid to liquid ratio, extraction time and temperature) on starch content, total phenolic content (TPC) and antioxidant activity of extracts obtained from seed kernel of mango var. Kesar. Isolation of starch from mango kernel by employing the ethanol precipitation method has been assessed. Box-Behnken design has been used to develop, validate and optimize the statistical model in order to establish the impact of various parameters either alone or in combination followed by characterization of starch. A decrease in pH has increased the extraction of starch while other responses such as TPC and antioxidant activity did not show a significant variation. The yield of total phenolic compound has been improved with decrease in solid to liquid ratio by increasing the solvent volume. The optimum conditions have been found to be 2.5 pH, 24 min extraction time and 1:55 g/mL solid to liquid ratio. Under these conditions, the highest extraction yield of starch and TPC are found to be 13.93 g/100 g and 33.45 mg GAE/g, respectively. No significant variation in antioxidant activity has been observed. A better and value-added utilization of the waste material can tender environmentally sustainable and economically viable solution.