通过基因图谱和小鼠中和试验检测产气荚膜梭菌类型。

Q3 Veterinary

BULGARIAN JOURNAL OF VETERINARY MEDICINE Pub Date : 2023-01-01 DOI:10.15547/bjvm.2021-0060

L. Abdolmohammadi Khiav, A. Zahmatkesh, A. Paradise

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引用次数: 0

摘要

本研究的目的是利用PCR和小鼠中和试验(MNT)检测产气荚膜荚膜杆菌的类型，并找到从伊朗动物样本中分离的毒力因子tpeL和netB的遗传谱。为此，采用MNT对46株产气荚膜荚膜杆菌进行了检测。采用16S rRNA-PCR和多重PCR对α、β、epsilon和iota毒素进行检测。对于tpeL和netB基因分析，分别进行PCR实验。MNT检测结果均为a型产气荚膜荚膜杆菌，16SrRNA-PCR和多重pcr检测结果一致。根据所得结果，分子检测与MNT的一致性为100%。tpeL和netB基因阳性率分别为52.1%和60.8%。总的来说，在A型产气荚膜荚膜原分离株中发现了4种遗传毒力因子谱。

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Detection of Clostridium perfringens types through genetic profiling and mouse neutralisation test.

The aim of this study was to detect the types of C. perfringens using PCR and mouse neutralisation test (MNT) and find the genetic profile of virulence factors tpeL and netB isolated from animal samples in Iran. For this purpose, 46 C. perfringens isolates were tested using MNT. Detection was also performed using 16S rRNA-PCR and multiplex PCR for alpha, beta, epsilon and iota toxins. For tpeL and netB gene profiling, separate PCR experiments were carried out. MNT detected that all isolates were C. perfringens type A. The same results were obtained in 16SrRNA-PCR and multiplex-PCR. Based on the obtained results, the molecular test was 100% consistent with MNT. Samples were positive for tpeL and netB genes in 52.1% and 60.8% isolates, respectively. Overall, four genetic virulence factor profiles were found in C. perfringens type A isolates.

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来源期刊

BULGARIAN JOURNAL OF VETERINARY MEDICINE Veterinary-Veterinary (all)

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

15 weeks

期刊介绍： BJVM is a no-fee open-access scientific quarterly journal which covers topics related to both fundamental and applied aspects of veterinary medicine and to closely connected subjects with it. The journal publishes original papers, short communications and reviews.