{"title":"无载流子[125I]IodoHoechst 33258的制备","authors":"Roger F. Martin, Marshall Pardee","doi":"10.1016/0020-708X(85)90049-3","DOIUrl":null,"url":null,"abstract":"<div><p>The DNA ligand Hoechst 33258 has been iodinated with carrier-free [<sup>125</sup>I]iodide by the lactoperoxidase method. The product was separated from other iodination products and the uniodinated compound by preparative thin layer chromatography. <sup>125</sup>I-labelled Hoechst 33258 is useful as a probe with DNA molecules of defined sequence since the decay of the <sup>125</sup>I atom results in the induction of a double-strand break, the location of which can be detected by DNA-sequencing techniques.</p></div>","PeriodicalId":22517,"journal":{"name":"The International journal of applied radiation and isotopes","volume":"36 9","pages":"Pages 745-747"},"PeriodicalIF":0.0000,"publicationDate":"1985-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-708X(85)90049-3","citationCount":"15","resultStr":"{\"title\":\"Preparation of carrier free [125I]IodoHoechst 33258\",\"authors\":\"Roger F. Martin, Marshall Pardee\",\"doi\":\"10.1016/0020-708X(85)90049-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The DNA ligand Hoechst 33258 has been iodinated with carrier-free [<sup>125</sup>I]iodide by the lactoperoxidase method. The product was separated from other iodination products and the uniodinated compound by preparative thin layer chromatography. <sup>125</sup>I-labelled Hoechst 33258 is useful as a probe with DNA molecules of defined sequence since the decay of the <sup>125</sup>I atom results in the induction of a double-strand break, the location of which can be detected by DNA-sequencing techniques.</p></div>\",\"PeriodicalId\":22517,\"journal\":{\"name\":\"The International journal of applied radiation and isotopes\",\"volume\":\"36 9\",\"pages\":\"Pages 745-747\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1985-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0020-708X(85)90049-3\",\"citationCount\":\"15\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The International journal of applied radiation and isotopes\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0020708X85900493\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The International journal of applied radiation and isotopes","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0020708X85900493","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Preparation of carrier free [125I]IodoHoechst 33258
The DNA ligand Hoechst 33258 has been iodinated with carrier-free [125I]iodide by the lactoperoxidase method. The product was separated from other iodination products and the uniodinated compound by preparative thin layer chromatography. 125I-labelled Hoechst 33258 is useful as a probe with DNA molecules of defined sequence since the decay of the 125I atom results in the induction of a double-strand break, the location of which can be detected by DNA-sequencing techniques.
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