{"title":"玉米植株对镰刀菌抗性的PCR评价","authors":"Lidia Tumanova, Cristina Grajdieru, Valentin Mitin, Irina Mitina","doi":"10.59295/sum1(171)2023_17","DOIUrl":null,"url":null,"abstract":"Fungal contamination of maize fields with Fusarium species leads to yield loss and affects grain quality. In this investigation, maize of three genotypes Ku123, RF7, MK01 at the silking, dough and physiological maturity stage were analyzed for mixed contamination with F. verticillioides, F. oxysporum, F.avenaceum. Asymptomatic mixed infection with F. oxysporum, F. verticillioides, F. avenaceum was detected in all samples of Ku123 and in some samples of the genotype RF7. The genotype MK01 was infected only with F. oxysporum. PCR estimation of the number of copies of Fusarium spp by serial dilutions demonstrated that genotype Ku123 is the most susceptible to the infection.","PeriodicalId":30698,"journal":{"name":"Studia Universitatis Moldaviae Stiinte reale si ale naturii","volume":"5 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of maize plant resistance to Fusarium species by PCR\",\"authors\":\"Lidia Tumanova, Cristina Grajdieru, Valentin Mitin, Irina Mitina\",\"doi\":\"10.59295/sum1(171)2023_17\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Fungal contamination of maize fields with Fusarium species leads to yield loss and affects grain quality. In this investigation, maize of three genotypes Ku123, RF7, MK01 at the silking, dough and physiological maturity stage were analyzed for mixed contamination with F. verticillioides, F. oxysporum, F.avenaceum. Asymptomatic mixed infection with F. oxysporum, F. verticillioides, F. avenaceum was detected in all samples of Ku123 and in some samples of the genotype RF7. The genotype MK01 was infected only with F. oxysporum. PCR estimation of the number of copies of Fusarium spp by serial dilutions demonstrated that genotype Ku123 is the most susceptible to the infection.\",\"PeriodicalId\":30698,\"journal\":{\"name\":\"Studia Universitatis Moldaviae Stiinte reale si ale naturii\",\"volume\":\"5 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Studia Universitatis Moldaviae Stiinte reale si ale naturii\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.59295/sum1(171)2023_17\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Studia Universitatis Moldaviae Stiinte reale si ale naturii","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.59295/sum1(171)2023_17","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Evaluation of maize plant resistance to Fusarium species by PCR
Fungal contamination of maize fields with Fusarium species leads to yield loss and affects grain quality. In this investigation, maize of three genotypes Ku123, RF7, MK01 at the silking, dough and physiological maturity stage were analyzed for mixed contamination with F. verticillioides, F. oxysporum, F.avenaceum. Asymptomatic mixed infection with F. oxysporum, F. verticillioides, F. avenaceum was detected in all samples of Ku123 and in some samples of the genotype RF7. The genotype MK01 was infected only with F. oxysporum. PCR estimation of the number of copies of Fusarium spp by serial dilutions demonstrated that genotype Ku123 is the most susceptible to the infection.
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