对猴头菌属大型菌培养滤液和菌丝的抑菌活性进行了研究

IF 0.9 4区 生物学 Q4 PLANT SCIENCES
Margarita Lomberg, Tetiana Krupodorova, Viktoriia Krasinko, Оksana Mykchaylova
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引用次数: 0

摘要

本研究的目的是研究从乌克兰国家科学院M.G. Kholodny植物学研究所IBK蘑菇培养收集的担子菌属Hericium属菌株的抗菌活性。共调查abietis、H. cirrhatum、H. coralloides、H. erinaceus等14种菌株。菌株在葡萄糖-蛋白胨-酵母菌液培养基上培养。通过圆盘扩散法和圆筒法对这些真菌的匀浆菌丝体和滤液进行革兰氏阳性(枯草芽孢杆菌、黄体微球菌和金黄色葡萄球菌)和革兰氏阴性(大肠杆菌和铜绿假单胞菌)细菌的抗性评估。菌株的活性变化显著。根据技术测定、菌种、菌株和真菌发育阶段的不同,被测细菌的抑制带范围为5.3 mm至20.0 mm。总的来说,所研究物种的培养滤液的抗菌潜力显著高于其菌丝活性。匀浆菌丝体仅对大肠杆菌具有潜在的良好效果。首次观察到abietis和cirrhatum两种菌株的抑菌活性。据我们所知,H. coralloides抑制铜绿假单胞菌和金黄色葡萄球菌生长的能力以前还没有报道过。研究结果表明,所研究的猴头菌种类能够产生具有宽谱和窄谱作用的抗菌代谢物,可能具有潜在的健康益处,可为进一步分析、分离和鉴定有潜力的药理学抗菌化合物提供参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The antibacterial activity of culture filtrates and mycelia of selected strains of macromycetes from the genus Hericium
The aim of the study was to investigate the antibacterial activity of selected strains of the genus Hericium, belonging to basidiomycetes, from the IBK Mushroom Culture Collection of the M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine. A total of 14 strains including H. abietis, H. cirrhatum, H. coralloides, and H. erinaceus were investigated. The strains were cultivated on a liquid glucose-peptone-yeast medium. Both the homogenised mycelium and filtrate of these fungi were evaluated against gram-positive (Bacillus subtilis, Micrococcus luteus, and Staphylococcus aureus) and gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria by the disk diffusion and cylinder methods. The activity of the strains varied significantly. Depending on the techniques assay, species, strain, and stage of fungal development, the inhibition zone of the tested bacteria ranged from 5.3 mm to 20.0 mm. In general, the antibacterial potential of the culture filtrates of the investigated species was significantly higher than their mycelia activity. The homogenised mycelium showed potentially good results only against E. coli. The antibacterial activities of the H. abietis and H. cirrhatum species were observed for the first time. To the best of our knowledge, the ability of H. coralloides to inhibit the growth of P. aeruginosa and S. aureus has not been previously reported. The obtained results indicate the ability of the studied Hericium species to produce antibacterial metabolites with a wide and narrow spectrum of action which might have potential health benefits and could be recommended for the further analysis, isolation and identification of potentially promising antibacterial compounds in pharmacology.
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来源期刊
Botanica Serbica
Botanica Serbica Agricultural and Biological Sciences-Plant Science
CiteScore
1.40
自引率
12.50%
发文量
17
审稿时长
34 weeks
期刊介绍: Botanica Serbica publishes original research papers on all aspects of plant, fungal and microbial biology research including the disciplines of microbiology, mycology, lichenology, bryology, flora, vegetation, biogeography, systematics, taxonomy, plant biotechnology, plant cell biology, plant ecology, environmental plant biology, forestry, genomics, horticulture, limnology, metabolomics, molecular biology, proteomics, virology, plant conservation and protection, and wildlife and ecosystem management.
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