Baoshan Cui, Jie Wang, Fei Huang, Nan Li, Qinghua Gao
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RNA-Seq study of in vivo-produced single male and female bovine blastocysts
The objective of this study was to identify differentially expressed genes (DEG) between females and males in early bovine embryos. Superovulated cattle (n = 4) in the age span of 16–18 months were artificially inseminated with semen from the same bull that has been proven to be fertile. Blastocysts were collected by routine non-surgical uterine flushing on day 7 after insemination. This study determines the sex of embryos using a micro-injection pipette to collect a few blastomeres through the [...]
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