Monique Ane da Luz Ferreira, Oscar Alexander Aguirre-Obando, Adriana Lacerda Twerdochlib, Angela Maria Palacio-Cortés, Mário Antônio Navarro-Silva
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引用次数: 0
摘要
埃及伊蚊是登革热、黄热病、基孔肯雅热和寨卡病毒的重要媒介。众所周知，抗性监测和遗传多样性数据有助于设计病媒控制方案。本研究旨在通过对来自巴西paran地区的6个埃及伊蚊自然种群的kdr突变Val1016IIe和F1534C的频率以及线粒体基因ND4的遗传变异，评估其对拟除虫菊酯类杀虫剂(PYs)的抗性。成虫卵采自南阿尔沃拉达、马里莱纳、马林、新隆德里纳、Paranavaí和 o Carlos do Ivaí。从这些成年人中，345人用于鉴定1016和1534个位点，120人用于进行ND4基因分析。研究群体表现出对PYs的抗性、低基因流和遗传多样性。此外，在来自亚马逊和巴西东南部、秘鲁、墨西哥和北美的人群中观察到单倍型之间的关系。
Genetic diversity and Kdr mutations of natural Aedes (Stegomyia) aegypti (Diptera: Culicidae) populations of Brazil
Aedes (Stegomyia) aegypti is an important vector of dengue, yellow fever, chikungunya and Zika virus. It is well known that resistance monitoring and genetic diversity data help designing the vector control programs. This study aimed to evaluate resistance to pyrethroids (PYs) through the frequency of kdr mutations Val1016IIe and F1534C, and the genetic variation of the mitochondrial gene ND4 in six natural populations of A. aegypti from Paraná - Brazil. Adults were obtained from eggs collected from Alvorada do Sul, Marilena, Maringá, Nova Londrina, Paranavaí and São Carlos do Ivaí. From these adults, 345 were used to identify the 1016 and 1534 sites, and 120 were used to perform the ND4 gene analysis. The studied populations from Paraná showed PYs resistance, low gene flow and genetic diversity. Additionally, a relationship was observed among the haplotypes of populations from the Amazon and Southeastern Brazil, Peru, Mexico, and North America.
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