俄勒冈素对肠道、肝脏和血浆微生物群及氨基酸基形成的影响

I. V. Nikalayeva, V. M. Sheibak, O. B. Astrouskaya, E. M. Doroshenko, V. Yu. Smirnov
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引用次数: 0

摘要

背景。本文分析了一个过程灌胃俄勒冈蛋白对肠壁粘蛋白微生物病、肠黏膜形态结构以及小肠、肝脏和血浆微生物组织复合物氨基酸基形成的影响。目标。该研究的目的是确定俄勒冈蛋白在正常动物体内的生物活性机制。材料和方法。实验对象为体重180 ~ 240 g的雌性白鼠。用0.005%的俄勒冈素水溶液,以5 mg/kg体重的剂量肠内给药,连续10天。对照组给予等量生理盐水。在小肠、肝脏和血浆的微生物组织复合物样品中,用高效液相色谱法测定游离氨基酸及其含氮代谢物的浓度。形态学和微生物学研究按标准方法进行。结果。在用俄勒冈素治疗的动物中,空肠壁的结构没有负面变化。在壁粘蛋白的微生物组中,由于双歧杆菌和乳酸菌的生长,观察到厌氧菌的数量增加。同时,还记录了对乳糖阴性菌群和产气菌群的抑菌作用。在小肠微生物组织复合体中,经肠内给药俄勒冈蛋白后,主要由兼性厌氧菌群产生的氨基酸和含氮衍生物的总水平降低。在肝脏中,摄入俄勒冈蛋白导致氨基酸及其衍生物的总量增加。血浆中蛋白质氨基酸及其含氮衍生物的总含量也有所下降。结论。俄勒冈素在动物肠内注射时显示出很高的生物活性。肠道菌群的积极变化和粘蛋白的增加,一方面可能抑制兼性厌氧菌产生含氮产物,另一方面,启动游离氨基酸在组织中用于生物合成目的,同时阻止其分解代谢。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
EFFECT OF OREGONIN ON THE MICROBIOME AND FORMATION OF THE AMINO ACID FUND OF THE INTESTINE, LIVER AND PLASMA
Background. This article analyzes the effect of a course intragastric administration of oregonin on the microbiocenosis of parietal mucin, the morphological structure of the intestinal mucosa, as well as the formation of the amino acid fund of the microbial-tissue complex of the small intestine, liver and plasma. Objective. The aim of the study was to identify the mechanisms of the biological activity of oregonin when administered to normal animals. Material and methods. The experiments were performed on white female rats weighing 180-240 g. The animals were enterally administered with 0.005% aqueous solution of oregonin at a dose of 5 mg/kg of body weight for 10 days daily. The control group received equivalent volumes of saline. In samples of the microbial-tissue complex of the small intestine, liver and plasma, the concentrations of free amino acids and their nitrogen-containing metabolites were determined by HPLC. Morphological and microbiological studies were carried out according to standard methods. Results. In animals treated with oregonin, there were no negative changes in the structure of the jejunal wall. In the microbiome of the parietal mucin, an increase in the population of anaerobes was observed, due to the growth of bifidobacteria and lactobacilli. At the same time, a bacteriostatic effect was recorded against lactose-negative and gas -forming microflora. In the microbial-tissue complex of the small intestine, after the enteral administration of oregonin, the total levels of amino acids and nitrogen-containing derivatives, which are produced mainly by facultative anaerobic microflora, decreased. In the liver, the intake of oregonin led to an increase in the total amount of amino acids and their derivatives. The total content of proteinogenic amino acids and their nitrogen-containing derivatives also decreased in the blood plasma. Conclusion. Oregonin demonstrates a high biological activity when administered enterally to animals. Positive changes in the intestinal microbiome and increased production of mucin, on the one hand, probably inhibit the production of nitrogen-containing products by facultative anaerobes, and on the other hand, initiate the use of free amino acids for biosynthetic purposes in tissues, while preventing their catabolism.
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