使用头发样本监测抗逆转录病毒治疗依从性

Philomena Chepkirui, Elizabeth Kigondu, Margaret Nganga, Ouma Josephine Adhiambo, Bhavna Chohan, Musa Otieno Ngayo, Beatrice Irungu
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摘要

导论:测量血液和尿液等生物基质中的抗逆转录病毒药物浓度以前已用于监测依从性。不幸的是,它们只反映了取样后1至2天内服用的抗逆转录病毒药物剂量。由于毛发检测具有较长的检测窗口期,因此已成为确定某些药物(尤其是滥用药物)长期暴露的首选工具。目的:本研究评估毛发样本在治疗药物监测(TDM)中作为抗逆转录病毒治疗依从性指标的效用。方法:本研究使用奈韦拉平(NVP)多年,奈韦拉平是肯尼亚一线抗逆转录病毒治疗的组成部分。分别从234例和328例病毒学失败(病毒载量为1000拷贝/mL)和抑制病毒载量(1000拷贝/mL)的同意接受一线抗逆转录病毒治疗的HIV患者中获得匹配的头发和血液样本。采用液相色谱-串联质谱法(LC-MS/MS)测定ARV血浆和毛发浓度。结果:计算中位数;头发和血浆样品NVP水平的四分位数范围(IQR)分别为36.8ng/ mL和19.32ng/mL。头发中NVP水平与匹配的血浆样本之间无显著差异(Wilcoxon符号秩检验;Z = -0.93, P>0.05)。结论:该研究表明,分析头发中的抗逆转录病毒药物可以确定药物暴露情况,作为传统血浆药物分析的替代方法,特别是在我们的环境中,实验室设施和进行静脉切开术的熟练人员很少或缺乏。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Use of Hair Samples for Monitoring of Antiretroviral Therapy Adherence
Introduction: Measurement of antiretrovirals (ARVs) drug concentration in biological matrices such as blood and urine has been used previously for monitoring adherence. Unfortunately, they only reflect ARV doses taken within 1 to 2 days of sampling. Hair testing has become the most preferred tool to determine chronic exposure to some drugs, especially drugs of abuse, because of its long detection window. Objective: This study, evaluated the utility of hair samples in therapeutic drug monitoring (TDM) as an indicator of ART adherence. Methods: This study used nevirapine (NVP), an ARV integral component of the first line ART in Kenya, for many years. Matched hair and blood samples were obtained from 234 and 328 consenting HIV patients on first line ART with virologic failure (viral load >1000 copies/mL) and suppressed viral load (VL<1000 copies/mL) respectively. The ARV plasma and hair concentrations were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results: The calculated median; interquartile range (IQR) of NVP levels in hair and plasma samples were 36.8ng/ mL and 19.32ng/mL respectively. There was no significant difference between the level of NVP in hair and matched plasma samples (Wilcoxon signed rank test; Z = -0.93, P> 0.05). Conclusion: The study has demonstrated that analysis of ARV drugs in the hair can determine drug exposure as an alternative to conventional plasma drug analysis, especially in our settings where laboratory facilities and skilled personnel to do phlebotomy are few or lacking.
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