犊牛腹泻大肠埃希菌的病原分型及耐药性特征

IF 0.3 4区综合性期刊 Q4 MULTIDISCIPLINARY SCIENCES

Comptes Rendus De L Academie Bulgare Des Sciences Pub Date : 2023-10-01 DOI:10.7546/crabs.2023.09.13

Sukru Kirkan, Goksel Erbas, Hafize Dolgun, Ugur Parin, Suheyla Turkyilmaz, Serap Savasan, Yigit Seferoglu

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引用次数: 0

摘要

犊牛腹泻是造成经济损失的重要疾病，大肠杆菌是犊牛腹泻的主要致病菌。本研究共采集了100头腹泻犊牛的粪便样本。经16S rRNA PCR分析，52株大肠杆菌中有50株(96.15%)为分子型大肠杆菌。通过对50株大肠杆菌的分子病理分型研究，鉴定出21株(42.0%)大肠杆菌的病原型。50株大肠杆菌对阿莫西林-克拉维酸耐药率为34%，对环丙沙星耐药率为38%，对甲氧苄啶-磺胺甲恶唑耐药率为60%。26株(52%)大肠杆菌检出不同程度的耐药基因。综上所述，为本地区犊牛大肠杆菌腹泻的抗菌治疗流程提供了数据来源，同时也为文献带来了可以指导未来研究的结果。

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Pathotyping and Antibiotic Resistance Characteristics of Escherichia coli Isolates from Calf Diarrhea

Calf diarrhea is an important disease that causes economic losses and E. coli is the primary pathogen. In this study, stool samples were collected from a total of 100 calves with diarrhea. As a result of 16S rRNA PCR analysis, it was confirmed that 50 (96.15%) of 52 E. coli isolates were molecularly E. coli. As a result of molecular pathotyping studies of 50 E. coli isolates, the pathotypes of 21 (42.0%) E. coli strains were identified. E. coli (n = 50) isolates were found to be resistant to amoxicillin-clavulanate by 34%, to ciprofloxacin by 38%, and to trimethoprim-sulfamethoxazole by 60%. Different degrees of antimicrobial resistance genes were detected in 26 (52%) E. coli isolates. In conclusion, a data source was provided for the antimicrobial treatment procedure in calf diarrhea caused by E. coli in our region, and the results that could guide future studies were also brought to the literature.

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来源期刊

Comptes Rendus De L Academie Bulgare Des Sciences 综合性期刊-综合性期刊

CiteScore

0.60

自引率

33.30%

发文量

181

审稿时长

3-6 weeks

期刊介绍： Founded in 1948 by academician Georgy Nadjakov, "Comptes rendus de l’Académie bulgare des Sciences" is also known as "Доклади на БАН","Доклады Болгарской академии наук" and "Proceeding of the Bulgarian Academy of Sciences". If applicable, the name of the journal should be abbreviated as follows: C. R. Acad. Bulg. Sci. (according to ISO)