大麻二酚和芦荟提取物对人体细胞的生物活性:作为一种有前景的抗肺癌药物

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摘要

根据世界卫生组织的数据,癌症是与心脏病并列的最具影响力的死亡原因之一,每年导致约1000万人死亡,占所有死亡人数的20%。肺癌是全球男性和女性癌症相关死亡的主要原因。在一些癌症细胞系体外实验的临床前研究中,已经获得了一些结果,从芦荟植物中提取的芦荟提取物(AVE)和从大麻中提取的大麻二酚(CBD)可能对癌症有抗癌作用,但这些结果的可靠性需要更多的分析。本研究从多方面研究了AVE和CBD对人肺癌(A549和BEAS)细胞的细胞毒性和凋亡作用。肺癌的早期诊断约占15%,晚期或转移期约占85%。因此,尽管今天使用了靶向药物,但患者的5年生存率仅为5-10%。本研究以通用和比较的方式研究了AVE和CBD对人肺癌细胞A549和健康正常BEAS-2B细胞的细胞毒性和凋亡作用。采用xCELLigence RTCA系统和AnnexinV-FITC/PI细胞凋亡法检测AVE和CBD的细胞毒作用。在目前的研究中,我们发现AVE和CBD可能在肺癌细胞(A549)中比在非癌症人上皮肺细胞系BEAS-2B中激发相对较高的细胞死亡率,这可能是通过调节线粒体代谢来实现的。综上所述,我们的研究正在申请专利,表明CBD和AVE有潜力发展成为一种有效的新型抗肺癌药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cannabidiol and Aloe vera Extract to Human Cells: Bioactive Molecules as a Promising Anti-Lung Cancer Drug
According to World Health Organisation data, cancer is one of the most influential diseases that goes head to head with heart diseases in the ranking of causes of death, causing approximately 10 million deaths annually and accounting for 20% of all deaths. Lung cancer is a leading cause of cancer-related deaths globally for both men and women. In some pre-clinical studies in cancer cell line in vitro experiments, some results have been obtained that Aloe Vera Extract (AVE) obtained from Aloe vera plant and Cannabindiol (CBD) obtained from Cannabis sativa may have anticancer effect against cancer, but more analysis is required for the reliability of these results. In this study, the cytotoxic and apoptotic effects of AVE and CBD in human lung cancer (A549 and BEAS) cells were studied in a multifaceted manner. Diagnosis of lung cancer disease is approximately 15% in the early stage and 85% in the late stage or metastatic stage. Therefore, despite the use of targeted drugs today, the 5-year survival rate of patients can only be 5-10%. In this study, the cytotoxic and apoptotic effects of AVE and CBD on human lung cancer cells A549 and healthy normal BEAS-2B cells were studied in a versatile and comparative manner. Cytotoxic effect of AVE and CBD was evaluated by xCELLigence RTCA System and AnnexinV-FITC/PI Apoptosis Assay by Flow Cytometry. In the current investigation, we discovered that AVE and CBD might incite a relative higher ratio of cell death in lung cancer cells (A549) than in non-cancer human epithelial lung cell line BEAS-2B, which may be achieved through regulating mitochondrial metabolism. In summary, our research is under Patent submission and shows that CBD and AVE have the potential to develop into a potent new anti-lung cancer drug.
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