犬瘟热犬脑细胞外基质的组织病理学特征

IF 0.4 4区 农林科学 Q4 VETERINARY SCIENCES
L.A. Oliveira, E.A. Viscone, A.A. Medeiros-Ronchi, M.B. Bandarra
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引用次数: 0

摘要

犬瘟热引起脱髓鞘性白质脑炎,类似于人类多发性硬化症。脑微环境,包括细胞外基质,是这种病变的一个重要关键因素,在多发性硬化症中已经有描述,但在犬瘟热中尚未得到证实。因此,这项工作的目的是表征脑细胞外基质与犬瘟热的狗。14只自然感染犬瘟热病毒的狗的皮质和小脑样本被送到乌伯拉尔印度联邦大学兽医医院的动物病理学实验室进行尸检后收集。样品按常规处理,用苏木精和伊红(H.E)、马松三色(MT)、周期性酸希夫(PAS)和Reticulin染色,然后进行描述。以染色百分率量化脱髓鞘和坏死区域。TM标本显示血管和脑膜周围有蓝色染色,表明病变区域胶原蛋白沉积较多。在坏死区域,网状蛋白染色显示血管壁紊乱,巨噬细胞内可见pas染色的粉红色颗粒。我们得出结论,细胞外基质似乎参与了犬瘟热的发病过程。应该做更多的研究,以更好地详细说明这些分子在这种疾病的过程中所起的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Histopathological features of the brain extracellular matrix from dogs with canine distemper
ABSTRACT Canine distemper causes demyelinating leucoencephalitis, like human multiple sclerosis. The encephalic microenvironment, including the extracellular matrix, is an important key factor of this lesion, already described in multiple sclerosis but not proved in canine distemper. Thereby, the aim of this work is to characterize the extracellular matrix in the encephalon of dogs with canine distemper. Samples of cortex and cerebellum of 14 naturally infected dogs with canine distemper virus were collected after being sent for necropsy in the Animal Pathology Laboratory of the Veterinary Hospital of Uberlândia Federal University. The samples were processed as routine, stained with Hematoxylin and Eosin (H.E), Masson Trichrome (MT), Periodic Acid-Schiff (PAS) and Reticulin, and then described. Areas of demyelination and necrosis were quantified in percentage of stain. The TM samples showed blue stain around vessels and meninge, which indicates a higher deposition of collagen in lesioned areas. At necrotic areas, reticulin stain pointed to a disorganization in the vascular wall and PAS-stained pink granules in macrophages. We conclude that the extracellular matrix seems to participate in the pathogeny of canine distemper. More research should be done to better detail the involvement of these molecules in the course of this disease.
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来源期刊
CiteScore
0.80
自引率
25.00%
发文量
111
审稿时长
9-18 weeks
期刊介绍: Publica artigos originais de pesquisa sobre temas de medicina veterinária, zootecnia, tecnologia e inspeção de produtos de origem animal e áreas afins relacionadas com a produção animal. Atualmente a revista mantém 628 permutas (419 internacionais e 209 nacionais), sendo um verdadeiro suporte para o recebimento de periódicos pela Biblioteca da Escola. A partir de 1999, a Escola de Veterinária delegou à FEP MVZ Editora o encargo do gerenciamento e edição de todas suas publicações, inclusive do Arquivo, ficando somente com o apoio logístico (instalações, equipamentos, pessoal etc.). O apoio financeiro é exercido pelo CNPq/FINEP e pela própria FEP MVZ.
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