Juliana da Silveira, Natália de Almeida Corrêa Alfredo, Marcia Regina Beux, Sila Mary Rodrigues Ferreira, Diego Averaldo Guiguet Leal
{"title":"含有甘氨酸的蜂胶水醇溶液对绿叶蔬菜中微生物和蠕虫卵联合还原的评价","authors":"Juliana da Silveira, Natália de Almeida Corrêa Alfredo, Marcia Regina Beux, Sila Mary Rodrigues Ferreira, Diego Averaldo Guiguet Leal","doi":"10.1590/1981-6723.04223","DOIUrl":null,"url":null,"abstract":"Abstract The main goal of this study was to evaluate the effectiveness of a propolis hydroalcoholic solution with glycine on the reduction of microorganisms and helminth eggs removal in lettuce leaves. Three experimental groups were evaluated in triplicate or replicates: microbiological (ME), parasitological (PE), and microbiological and parasitological (MPE). Before the experiments, the initial concentration of the native microbiota was assessed by counting mesophilic aerobes, molds and yeasts, using conventional microbiological techniques. Lettuce leaves were washed in tap water and subjected to different immersion treatments: propolis hydroalcoholic solution (PHS), propolis hydroalcoholic solution at pH 5.5 (PHS2), and propolis hydroalcoholic solution with glycine (PHS-glycine). In ME, propolis hydroalcoholic solution combined or not with glycine, was evaluated on the reduction of native microbiota. Residual microbiological contamination was assessed. In the PE, the effect of PHS2 and PHS-glycine was evaluated on the removal of a known number of Ascaris suum eggs on leaves artificially contaminated. In MPE, PHS-glycine was evaluated on the reduction of native microbiota and removal of eggs. A reduction of more than 2 log CFU/g was observed in ME. In PE, PHS-glycine removed 37.22% of eggs. In MPE, the PHS-glycine reduced 3.3 CFU/g of mesophilic aerobes, 5.0 log CFU/g of fungi, and 35.53% of eggs from vegetables. The results highlighted the solution’s efficiency in reducing or eliminating different contaminants in vegetables and reinforced the need for the development of hygiene methods that do not harm human health and are reproducible at the household level.","PeriodicalId":9112,"journal":{"name":"Brazilian Journal of Food Technology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of a propolis hydroalcoholic solution with glycine in the joint reduction of microorganisms and helminth eggs from leafy green vegetables\",\"authors\":\"Juliana da Silveira, Natália de Almeida Corrêa Alfredo, Marcia Regina Beux, Sila Mary Rodrigues Ferreira, Diego Averaldo Guiguet Leal\",\"doi\":\"10.1590/1981-6723.04223\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract The main goal of this study was to evaluate the effectiveness of a propolis hydroalcoholic solution with glycine on the reduction of microorganisms and helminth eggs removal in lettuce leaves. Three experimental groups were evaluated in triplicate or replicates: microbiological (ME), parasitological (PE), and microbiological and parasitological (MPE). Before the experiments, the initial concentration of the native microbiota was assessed by counting mesophilic aerobes, molds and yeasts, using conventional microbiological techniques. Lettuce leaves were washed in tap water and subjected to different immersion treatments: propolis hydroalcoholic solution (PHS), propolis hydroalcoholic solution at pH 5.5 (PHS2), and propolis hydroalcoholic solution with glycine (PHS-glycine). In ME, propolis hydroalcoholic solution combined or not with glycine, was evaluated on the reduction of native microbiota. Residual microbiological contamination was assessed. In the PE, the effect of PHS2 and PHS-glycine was evaluated on the removal of a known number of Ascaris suum eggs on leaves artificially contaminated. In MPE, PHS-glycine was evaluated on the reduction of native microbiota and removal of eggs. A reduction of more than 2 log CFU/g was observed in ME. In PE, PHS-glycine removed 37.22% of eggs. In MPE, the PHS-glycine reduced 3.3 CFU/g of mesophilic aerobes, 5.0 log CFU/g of fungi, and 35.53% of eggs from vegetables. The results highlighted the solution’s efficiency in reducing or eliminating different contaminants in vegetables and reinforced the need for the development of hygiene methods that do not harm human health and are reproducible at the household level.\",\"PeriodicalId\":9112,\"journal\":{\"name\":\"Brazilian Journal of Food Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brazilian Journal of Food Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1590/1981-6723.04223\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Food Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/1981-6723.04223","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Evaluation of a propolis hydroalcoholic solution with glycine in the joint reduction of microorganisms and helminth eggs from leafy green vegetables
Abstract The main goal of this study was to evaluate the effectiveness of a propolis hydroalcoholic solution with glycine on the reduction of microorganisms and helminth eggs removal in lettuce leaves. Three experimental groups were evaluated in triplicate or replicates: microbiological (ME), parasitological (PE), and microbiological and parasitological (MPE). Before the experiments, the initial concentration of the native microbiota was assessed by counting mesophilic aerobes, molds and yeasts, using conventional microbiological techniques. Lettuce leaves were washed in tap water and subjected to different immersion treatments: propolis hydroalcoholic solution (PHS), propolis hydroalcoholic solution at pH 5.5 (PHS2), and propolis hydroalcoholic solution with glycine (PHS-glycine). In ME, propolis hydroalcoholic solution combined or not with glycine, was evaluated on the reduction of native microbiota. Residual microbiological contamination was assessed. In the PE, the effect of PHS2 and PHS-glycine was evaluated on the removal of a known number of Ascaris suum eggs on leaves artificially contaminated. In MPE, PHS-glycine was evaluated on the reduction of native microbiota and removal of eggs. A reduction of more than 2 log CFU/g was observed in ME. In PE, PHS-glycine removed 37.22% of eggs. In MPE, the PHS-glycine reduced 3.3 CFU/g of mesophilic aerobes, 5.0 log CFU/g of fungi, and 35.53% of eggs from vegetables. The results highlighted the solution’s efficiency in reducing or eliminating different contaminants in vegetables and reinforced the need for the development of hygiene methods that do not harm human health and are reproducible at the household level.
期刊介绍:
The Brazilian Journal of Food Technology (BJFT) is an electronic rolling pass publication with free access, whose purpose is to publish unpublished articles based on original research results and technological information that significantly contribute to the dissemination of new knowledge related to production and evaluation of food in the areas of science, technology, food engineering and nutrition (non-clinical). Manuscripts of national or international scope are accepted, presenting new concepts or experimental approaches that are not only repositories of scientific data. The Journal publishes original articles, review articles, scientific notes, case reports, and short communication in Portuguese and English. The submission of a manuscript presupposes that the same paper is not under analysis for publication in any other divulging vehicle. Articles specifically contemplating analytical methodologies will be accepted as long as they are innovative or provide significant improvement to existing methods. It is at the discretion of the Editors, depending on the subject relevance, the acceptance of works with test results of industrialized products without the information necessary to manufacture them. Papers aimed essentially at commercial propaganda will not be accepted.