高轴向分辨率的荧光干涉结构照明显微镜三维形态学成像

IF 20.6 1区物理与天体物理 Q1 OPTICS

Advanced Photonics Pub Date : 2023-09-19 DOI:10.1117/1.ap.5.5.056007

Yile Sun, Hongfei Zhu, Lu Yin, Hanmeng Wu, Mingxuan Cai, Weiyun Sun, Yueshu Xu, Xinxun Yang, Jiaxiao Han, Wenjie Liu, Yubing Han, Xiang Hao, Renjie Zhou, Cuifang Kuang, Xu Liu

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引用次数: 0

摘要

高轴向分辨率的三维亚细胞结构成像一直是荧光显微镜的核心目的。然而，轴向分辨率和其他重要技术指标(如时间分辨率、光功率密度和成像过程复杂性)之间存在权衡。我们报道了一种新的成像方式，荧光干涉结构照明显微镜(FI-SIM)，它是基于三维结构照明显微镜进行宽视场横向成像和荧光干涉进行轴向重建。FI-SIM可以在数百毫秒的数量级内快速获取图像，并且在没有z轴扫描的情况下，在一半的波长深度范围内显示甚至30纳米的轴向分辨率。此外，相对较低的激光功率密度放宽了对染料的要求，使观察固定和活的亚细胞结构的应用范围广泛。

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Fluorescence interference structured illumination microscopy for 3D morphology imaging with high axial resolution

Imaging three-dimensional, subcellular structures with high axial resolution has always been the core purpose of fluorescence microscopy. However, trade-offs exist between axial resolution and other important technical indicators, such as temporal resolution, optical power density, and imaging process complexity. We report a new imaging modality, fluorescence interference structured illumination microscopy (FI-SIM), which is based on three-dimensional structured illumination microscopy for wide-field lateral imaging and fluorescence interference for axial reconstruction. FI-SIM can acquire images quickly within the order of hundreds of milliseconds and exhibit even 30 nm axial resolution in half the wavelength depth range without z-axis scanning. Moreover, the relatively low laser power density relaxes the requirements for dyes and enables a wide range of applications for observing fixed and live subcellular structures.

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来源期刊

Advanced Photonics OPTICS-

CiteScore

22.70

自引率

1.20%

发文量

审稿时长

18 weeks

期刊介绍： Advanced Photonics is a highly selective, open-access, international journal that publishes innovative research in all areas of optics and photonics, including fundamental and applied research. The journal publishes top-quality original papers, letters, and review articles, reflecting significant advances and breakthroughs in theoretical and experimental research and novel applications with considerable potential. The journal seeks high-quality, high-impact articles across the entire spectrum of optics, photonics, and related fields with specific emphasis on the following acceptance criteria: -New concepts in terms of fundamental research with great impact and significance -State-of-the-art technologies in terms of novel methods for important applications -Reviews of recent major advances and discoveries and state-of-the-art benchmarking. The journal also publishes news and commentaries highlighting scientific and technological discoveries, breakthroughs, and achievements in optics, photonics, and related fields.