水稻抗旱性和产量基因型筛选及变异研究

Sheeba A, Tamil Selvi C, Yogameenakshi P, Bhaskaran M, Banumathy S
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摘要

本研究对9个水稻基因型(TM 12061、TM 12077、TM 12012、TM 14035、TM 16017、Senthuram、Vandhana、TKM 12和Anna (R) 4)进行了室内和田间筛选,以评价其抗旱潜力。在实验室筛选中,研究了不同PEG浓度(-0.2、-0.4、-0.6和-1.0 MPa)对发芽、茎长和根长的影响。随着PEG浓度的增加,各基因型的萌发势均显著降低。培养物TM 12077对PEG诱导的干旱胁迫表现出较高的耐受性,在较高的PEG浓度(1.0 MPa)下,茎长为3.2 cm、根长为8.85 cm,发芽率为30.8%。在管理胁迫条件下的田间筛选中,培养物TM 12077和TM 12012的脯氨酸积累量较高(4.15 mg/g)。TM 12012、TM 12077和TM 12061基因型叶绿素稳定性指数均在80%以上。培养物TM 14035、品种Anna (R) 4和Vandhana在115天内早熟。在管理胁迫条件下,Vandhana (103.3 cm)、TM 12077(105.4 cm)、TM 12061 (108.2 cm)和TM 12012 (110.5 cm)株高适中。单株分蘖数、穗数/ m2和产量/公顷均以栽培tm12077为最高。通过室内和田间筛选,发现TM 12077、TM 12012和TM 12061具有良好的抗旱性,可作为水稻抗旱性育种的供体。在株高、单株分蘖数、叶绿素稳定性指数和总叶绿素含量方面,均有较高的遗传力和中至高的遗传率,表明存在加性基因效应和通过直接选择对其进行改进的空间。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Screening and Variability Studies in Rice Genotypes for Drought Tolerance and Yield
In the present investigation, laboratory and field screening of nine rice genotypes, namely, TM 12061, TM 12077, TM 12012, TM 14035, TM 16017, Senthuram, Vandhana, TKM 12 and Anna (R) 4 was taken up to assess their drought tolerance potential. For laboratory screening, the effect of different levels of PEG concentration namely, -0.2, -0.4, -0.6 and -1.0 MPa on germination, shoot length and root length were studied. There was a considerable decrease in the germination potential among all the genotypes with increase in PEG concentration. The culture, TM 12077 showed higher level of tolerance to PEG induced drought stress showing 30.8% germination with 3.2 cm and 8.85 cm shoot and root length, respectively at higher level of PEG concentration (1.0 MPa). In field screening under managed stress condition, the cultures, TM 12077 and TM 12012 showed higher accumulation of proline (4.15 mg/g). Chlorophyll stability index was more than 80% in the genotypes, TM 12012, TM 12077, and TM 12061. The culture TM 14035, the varieties Anna (R) 4 and Vandhana matured early in 115 days. Plant height was found to be moderate in Vandhana (103.3 cm), TM 12077(105.4 cm), TM 12061 (108.2 cm) and TM 12012 (110.5 cm) under managed stress condition. The number of tillers per plant, number of panicles/sqm and yield/hectare were maximum in the culture, TM 12077 under managed stress condition. Based on laboratory and field screening, the cultures TM 12077, TM 12012, and TM 12061 were found promising for water stress environment and can be utilized as donors in the breeding programs for drought tolerance in rice. High heritability coupled with moderate to high GA as per cent of mean was recorded for plant height, tillers per plant, chlorophyll Stability index and total chlorophyll content indicating the presence of additive gene effects and scope for their improvement through direct selection.
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