Bojan Milovanović, Branislav Kureljušić, Vesna Milićević, Nemanja Zdravković, Jasna Kureljušić, Darko Marinković, Milan Maletić
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After detection of Mycoplasma spp. genome in uterine samples, the swabs of the vulva, vagina, nasal swab, and samples of milk were sampled in order to exclude possible contamination with other microorganisms as potential trigger of subclinical endometritis. The genome of Mycoplasma spp. in the uterus was confirmed in 4 cows (2.40%). Sequencing of the 16S RNA revealed that detected mycoplasmas belonged to Mycoplasma bovigenitalium and Mycoplasma tauri species, with a prevalence of 1.80% and 0.6%, respectively. The cytological evaluation showed a 69.90%, 54.87% and 48.33% of polymorphonuclear cells for M. bovigenitalium positive cows, and 61.64% for M. tauri positive cow. Bacteriological examination revealed Trueperella pyogenes in uterine samples in all 4 observed cows. This is the first study where M. tauri was detected in the uterus of a live cow with subclinical enodmetritis. 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引用次数: 0
摘要
摘要支原体是导致牛繁殖效率低下的严重健康疾病。支原体感染以慢性精囊炎、不孕症、外阴阴道炎、难产为主。本研究的目的是调查产后奶牛子宫支原体的频率及其在亚临床子宫内膜炎发生中的潜在作用。研究对象为102头产后22-32天的荷斯坦弗里西亚奶牛。使用cytopprint A I®对子宫样本进行细胞学、细菌学和分子分析。使用Metricheck®评估宫颈阴道粘液。在子宫样本中检测到支原体基因组后,对外阴拭子、阴道拭子、鼻拭子和牛奶样本进行取样,以排除其他微生物污染可能引发亚临床子宫内膜炎的可能性。4头奶牛(2.40%)子宫支原体基因组得到确认。16S RNA测序结果显示,检测到的支原体属于博维吉尼亚支原体和牛头支原体,患病率分别为1.80%和0.6%。细胞学鉴定结果显示,牛分枝杆菌阳性奶牛的多形核细胞阳性率分别为69.90%、54.87%和48.33%,牛头杆菌阳性奶牛的多形核细胞阳性率为61.64%。4头奶牛子宫标本细菌学检查均发现化脓性真芽孢杆菌。这是首次在患有亚临床子宫内膜炎的活牛的子宫中检测到牛头分枝杆菌的研究。本文推测牛头分枝杆菌和牛头分枝杆菌可能在亚临床子宫内膜炎的发病中起一定的作用。
Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in Holstein Friesian dairy cows with subclinical endometritis
Abstract Mycoplasma spp. is the cause of serious cattle health disorders that lead to poor reproductive efficiency. Chronic seminal vesiculitis, infertility, vulvovaginitis and dystocia were observed in Mycoplasma spp. infection. The objectives of the present study were to investigate the frequency o f Mycoplasmas in the uterus of postpartum dairy cows and its potential role in the occurrence of subclinical endometritis. Our study included 102 Holstein Friesian dairy cows 22-32 days postpartum. Uterine samples for cytological, bacteriological, and molecular analysis were provided using Cytoprint A I ® . Metricheck ® was used for the assessment of cervicovaginal mucus. After detection of Mycoplasma spp. genome in uterine samples, the swabs of the vulva, vagina, nasal swab, and samples of milk were sampled in order to exclude possible contamination with other microorganisms as potential trigger of subclinical endometritis. The genome of Mycoplasma spp. in the uterus was confirmed in 4 cows (2.40%). Sequencing of the 16S RNA revealed that detected mycoplasmas belonged to Mycoplasma bovigenitalium and Mycoplasma tauri species, with a prevalence of 1.80% and 0.6%, respectively. The cytological evaluation showed a 69.90%, 54.87% and 48.33% of polymorphonuclear cells for M. bovigenitalium positive cows, and 61.64% for M. tauri positive cow. Bacteriological examination revealed Trueperella pyogenes in uterine samples in all 4 observed cows. This is the first study where M. tauri was detected in the uterus of a live cow with subclinical enodmetritis. In this paper we hypothesized that M. bovigenitalium and M. tauri may have a certain role in the etiology of subclinical enodmetritis.
期刊介绍:
The Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research articles, invited review articles, and to limited extent methodology articles and case reports. The journal considers articles on all aspects of veterinary science and medicine, including the diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.