High-degree polymerizate IMOs of dextranase hydrolysates enhance Lactobacillus acid metabolism: Based on growth, and metabolomic and transcriptomic analyses

This study aimed to explore the growth and metabolic mechanisms and functional properties of the combination of high-degree polymerizate (DP) isomaltooligosaccharides (IMOs) and Lactobacillus to provide a basis for their subsequent applications in functional foods in improving human health. Metabolomic and transcriptomic analyses were used to select the important metabolic pathways, regulatory genes, and metabolites involved in the growth of Lactobacillus by high DP IMOs. Lactobacillus paracasei JLPF-176 grew well in the MRS medium with high DP IMOs. The best effect in promoting L. paracasei JLPF-176 biofilm formation was 58.94%. When high DP IMOs hydrolyzed for 48 h were added to the MRS medium, 684 genes were upregulated and 276 genes were downregulated in L. paracasei JLPF-176, in important pathways such as d-amino acid metabolism, tryptophan metabolism, arginine and proline metabolism. HMDB compound classification statistics revealed 421 organic acids and derivatives, accounting for the highest number of 28.39%. The number of genes annotated in amino acid metabolism was 107.