快速抗原检测能减轻检测实验室的负担吗?COVID-19大流行期间检测方法评价

Showkat Ahmad Lone, Tufail Ahmed, Umara Amin, Aashaq Hussain Allaie, Kowsar Jan, Amrit Pal Kour, Junaid Ahmad
{"title":"快速抗原检测能减轻检测实验室的负担吗?COVID-19大流行期间检测方法评价","authors":"Showkat Ahmad Lone, Tufail Ahmed, Umara Amin, Aashaq Hussain Allaie, Kowsar Jan, Amrit Pal Kour, Junaid Ahmad","doi":"10.7860/njlm/2023/59890.2688","DOIUrl":null,"url":null,"abstract":"Introduction: Timely diagnosis and isolation of cases is of paramount importance to contain the spread of a pandemic. The Coronavirus Disease-2019 (COVID-19) has emerged as a major health problem that needs concerted efforts for mitigation and control. Real-time Reverse Transcription Polymerase Chain Reaction (RT-PCR), the gold-standard diagnostic modality, has high cost and can be performed in special laboratories. Rapid Antigen Tests (RAT) has been developed to serve as an alternative and is recommended to use at point-of-care testing. Aim: To compare the case detection rate of RAT and RT-PCR and the possible role they may play in the pandemic mitigation efforts. Materials and Methods: In this retrospective study, all the samples collected during a nine-months period were analysed. Depending upon the criteria, either a RAT or RT- PCR was done on the samples. Data was analysed using descriptive statistics (frequencies, mean, standard deviation, and percentages). Results: A total of 8,29,745 samples were tested during the study period among which number of positive samples was 19,414 giving an overall positivity rate of 2.34% (0.20% to 12.58%). RAT positivity was 1.58% while RT-PCR gave a positivity of 4.26. Total number of positive cases identified by RAT and RT-PCR were 9,382 and 10,032, respectively. Conclusions: RAT is a low-cost alternative to the expensive RT-PCR with the added advantage of giving accurate and timely results. This can be a game changer especially in low-resource settings, which have witnessed a increase in the spread of COVID-19 during the latter part of the pandemic.","PeriodicalId":31115,"journal":{"name":"National Journal of Laboratory Medicine","volume":"28 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Can Rapid Antigen Tests Lessen the Burden on Testing Laboratories? An Evaluation of the Testing Methods during the COVID-19 Pandemic\",\"authors\":\"Showkat Ahmad Lone, Tufail Ahmed, Umara Amin, Aashaq Hussain Allaie, Kowsar Jan, Amrit Pal Kour, Junaid Ahmad\",\"doi\":\"10.7860/njlm/2023/59890.2688\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Introduction: Timely diagnosis and isolation of cases is of paramount importance to contain the spread of a pandemic. The Coronavirus Disease-2019 (COVID-19) has emerged as a major health problem that needs concerted efforts for mitigation and control. Real-time Reverse Transcription Polymerase Chain Reaction (RT-PCR), the gold-standard diagnostic modality, has high cost and can be performed in special laboratories. Rapid Antigen Tests (RAT) has been developed to serve as an alternative and is recommended to use at point-of-care testing. Aim: To compare the case detection rate of RAT and RT-PCR and the possible role they may play in the pandemic mitigation efforts. Materials and Methods: In this retrospective study, all the samples collected during a nine-months period were analysed. Depending upon the criteria, either a RAT or RT- PCR was done on the samples. Data was analysed using descriptive statistics (frequencies, mean, standard deviation, and percentages). Results: A total of 8,29,745 samples were tested during the study period among which number of positive samples was 19,414 giving an overall positivity rate of 2.34% (0.20% to 12.58%). RAT positivity was 1.58% while RT-PCR gave a positivity of 4.26. Total number of positive cases identified by RAT and RT-PCR were 9,382 and 10,032, respectively. Conclusions: RAT is a low-cost alternative to the expensive RT-PCR with the added advantage of giving accurate and timely results. This can be a game changer especially in low-resource settings, which have witnessed a increase in the spread of COVID-19 during the latter part of the pandemic.\",\"PeriodicalId\":31115,\"journal\":{\"name\":\"National Journal of Laboratory Medicine\",\"volume\":\"28 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"National Journal of Laboratory Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.7860/njlm/2023/59890.2688\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"National Journal of Laboratory Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7860/njlm/2023/59890.2688","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

导言:及时诊断和隔离病例对于遏制大流行的传播至关重要。2019冠状病毒病(COVID-19)已成为需要共同努力缓解和控制的重大健康问题。实时逆转录聚合酶链反应(RT-PCR)是金标准的诊断方法,但成本高,需要在专门的实验室进行。快速抗原检测(RAT)已被开发作为一种替代方法,并被建议用于即时检测。目的:比较RAT和RT-PCR的病例检出率及其在大流行缓解工作中的可能作用。材料与方法:回顾性分析9个月期间收集的所有样本。根据标准,对样品进行RAT或RT- PCR。使用描述性统计(频率、平均值、标准差和百分比)对数据进行分析。结果:研究期间共检测样本829745份,阳性样本19414份,总阳性率为2.34%(0.20% ~ 12.58%)。RAT阳性率为1.58%,RT-PCR阳性率为4.26%。RAT和RT-PCR检测的阳性病例总数分别为9382例和10032例。结论:RAT是昂贵的RT-PCR的低成本替代品,具有提供准确及时结果的额外优势。这可能会改变游戏规则,特别是在资源匮乏的环境中,在大流行后期,COVID-19的传播有所增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Can Rapid Antigen Tests Lessen the Burden on Testing Laboratories? An Evaluation of the Testing Methods during the COVID-19 Pandemic
Introduction: Timely diagnosis and isolation of cases is of paramount importance to contain the spread of a pandemic. The Coronavirus Disease-2019 (COVID-19) has emerged as a major health problem that needs concerted efforts for mitigation and control. Real-time Reverse Transcription Polymerase Chain Reaction (RT-PCR), the gold-standard diagnostic modality, has high cost and can be performed in special laboratories. Rapid Antigen Tests (RAT) has been developed to serve as an alternative and is recommended to use at point-of-care testing. Aim: To compare the case detection rate of RAT and RT-PCR and the possible role they may play in the pandemic mitigation efforts. Materials and Methods: In this retrospective study, all the samples collected during a nine-months period were analysed. Depending upon the criteria, either a RAT or RT- PCR was done on the samples. Data was analysed using descriptive statistics (frequencies, mean, standard deviation, and percentages). Results: A total of 8,29,745 samples were tested during the study period among which number of positive samples was 19,414 giving an overall positivity rate of 2.34% (0.20% to 12.58%). RAT positivity was 1.58% while RT-PCR gave a positivity of 4.26. Total number of positive cases identified by RAT and RT-PCR were 9,382 and 10,032, respectively. Conclusions: RAT is a low-cost alternative to the expensive RT-PCR with the added advantage of giving accurate and timely results. This can be a game changer especially in low-resource settings, which have witnessed a increase in the spread of COVID-19 during the latter part of the pandemic.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
71
审稿时长
12 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信