牛胚胎的低温保存。

R Falge, P Rommel, D Oesterreich, F Seifert, H Müller, U Freymann, B Draheim
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引用次数: 0

摘要

Niemann(1985)提出的用于牛胚胎移植的7天龄胚胎冷冻保存方法,在三个牛场和三个实验动物单位的生产条件下进行了测试。供体128例,获得467个完整胚胎,冷冻保存于精液吸管中。解冻后,回收了455个,其中439个(96.5%)适合转移。在412例移植中,妊娠率为49.0%。这一比例因母牛受者的排卵周期条件而异,7天胚胎受者的比例为46.0%,8天受者的比例为45.7%,6天受者的比例为65.8。与同一单位记录的7天新鲜胚胎移植妊娠结果相关,效率系数为0.69(550例新鲜移植= 65.4%,222例冷冻移植= 48.2%)。该方法推荐用于一般的野外实践。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Cryopreservation of bovine embryos].

The method of cryopreservation of embryos aged seven days, proposed for embryo transfer with cattle by Niemann (1985), was tested under production conditions on three cattle breeding farms and three experimental animal units. The number of donors was 128, and 467 intact embryos were obtained from them and were cryopreserved in semen straw. Following thawing, 455 were recovered, and 439 (96.5 percent) of these were suitable for transfer. A pregnancy rate of 49.0 percent was recorded from 412 transfers. This rate was differentiated by oestric cycle conditions of heifer recipients, which gave percentages of 46.0 among recipients of seven-day old embryos, 45.7 for eight-day recipients, and 65.8 for six-day recipients. Related to pregnancy results recorded on the same units from transfer of freshly collected seven-day embryos, the efficiency coefficient was 0.69 (550 fresh transfers = 65.4 percent and 222 cryopreserved transfers = 48.2 percent). The method is recommended for general field practice.

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