一种无模软光刻方法,用于使用光聚合物片材快速、低成本和批量制造微流控芯片

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION
R. Rahul, Nikhil Prasad, R. R. Ajith, P. Sajeesh, R. S. Mini, Ranjith S. Kumar
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引用次数: 0

摘要

现有的微流控芯片制造技术大多复杂、耗时、成本高,不适合批量生产。即将商业化的芯片实验室设备需要开发新的微加工方法,这些方法涉及的程序复杂性最小,使用成本效益高的材料。本文提出了一种廉价且省时的方法,用于在柔性版片上构建微流体装置,该柔性版片可作为商业现成材料使用,使用无模软光刻方法。微通道设计被转移到负阻光敏聚合物片(PPS)使用准直紫外线(UV)射线和蚀刻进行去除未暴露的材料。微通道网络在顶部由相同材料的光聚合物片密封,并在紫外线存在下进行压力辅助键合。配合表面的光聚合物之间的交联确保了相对较高的结合强度和完美的密封性。使用该方法创建了100-500 \(\upmu\) m宽度的简单和复杂微通道网络,并进行了各种表征测试。通过功能泄漏测试,确保制作的芯片能够承受200 kPa的压力,最大流量为12 mL/min。细胞培养,生物分子可视化和液滴混合动力学在微芯片的研究,以证明其实际用途。此外,还利用该三步法制作出了260 \(\times\) 190 mm \(^2\)的PPS大面积芯片。最重要的是,这种方法可以在2小时内批量生产24个具有多种设计的微芯片。换句话说,制造单个微芯片(50 \(\times\) 30 mm \(^2\))的平均时间不到5分钟。结果表明,这是一种有前途的方法,足够灵活,可以制造大尺寸芯片,并批量制造具有高保真度的多通道设计的微芯片。由于柔版基础设施和材料在资源有限的环境中非常便宜和常见,因此所提出的方法在芯片实验室设备快速商业化的背景下更为重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A mould-free soft-lithography approach for rapid, low-cost and bulk fabrication of microfluidic chips using photopolymer sheets

A mould-free soft-lithography approach for rapid, low-cost and bulk fabrication of microfluidic chips using photopolymer sheets

Most of the existing microfluidic chip fabrication techniques are very complex, time-consuming, costly, and are not amenable to mass manufacturing. Impending commercialization of lab-on-a-chip devices demand development of new microfabrication methods that involve least procedural complexities using cost-effective materials. This paper proposes an inexpensive and time-efficient procedure for constructing microfluidic devices on a flexographic sheet which is available as commercial-off-the-shelf material, using a mould-free soft-lithography approach. Microchannel design is transferred to a negative-resist photopolymer sheet (PPS) using collimated ultraviolet (UV) rays and etching is performed to remove unexposed material. The microchannel network is sealed on the top by a photopolymer sheet of the same material and pressure-assisted bonding is performed in the presence of UV. The cross-linking between photopolymers in the mating surfaces ensures relatively high bond strength and perfect sealing. Simple and complex microchannel network with 100–500 \(\upmu\)m width is created using this method and various characterization tests are performed. A functional leakage test ensured that the fabricated chip could withstand 200 kPa pressure at a maximum flow rate of 12 mL/min. Cell culture, biomolecule visualization, and droplet mixing dynamics are studied in the microchip to demonstrate its practical utility. Moreover, a large-area chip with 260 \(\times\) 190 mm\(^2\) is created using PPS with this three-step method. Most importantly, this method could mass produce 24 microchips with multiple designs within a span of 2 h. In other words, the average time incurred for the fabrication of a single microchip (50 \(\times\) 30 mm\(^2\)) is less than 5 min. Results suggest that it is a promising method flexible enough to create large-sized chips and to bulk-fabricate microchips having versatile channel designs with high fidelity. Since flexographic infrastructure and materials are very cheap and common in resource-limited settings, the proposed method assumes more importance in the context of rapid commercialization of lab-on-a-chip devices.

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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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