{"title":"利用issr指纹图谱和DNA测序对印度泰米尔纳德邦(Tamil nadu)不同种质的超彩花(gloriosa superba, l)进行种内分析","authors":"J. Jasmine, V. Balakrishnan","doi":"10.25081/RIPB.2018.V8.3600","DOIUrl":null,"url":null,"abstract":"Diversity within and among the population of Gloriosa superba collected from five different location of Tamil Nadu State, India, were explored by using Inter Simple Sequence Repeat (ISSR) DNA sequencing method. A total of 86.27% of polymorphic bands were seen in 94 reproducible bands generated from 12 number of ISSR primer ranging between 200bp to1000bp of band width. ISSR primer produces the bands with an average polymorphism of more than ninety nine percent among all the ecotypes. The dendrograms drawn for the analysis of genetic similarity in all the ecotypes. Our studies reveals that, genetic variations among different accessions of Gloriosa superba(L) was very well identified with the help of ISSR finger printings technic.","PeriodicalId":146947,"journal":{"name":"Research in Plant Biology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2018-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"INTRA SPECIFIC ANALYSIS OF GLORIOSA SUPERBA (L) THROUGH ISSR FINGER PRINTING AND DNA SEQUENCING OF ECOTYPES COLLECTED FROM DIFFERENT ACCESSIONS OF TAMIL NADU STATE, INDIA\",\"authors\":\"J. Jasmine, V. Balakrishnan\",\"doi\":\"10.25081/RIPB.2018.V8.3600\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Diversity within and among the population of Gloriosa superba collected from five different location of Tamil Nadu State, India, were explored by using Inter Simple Sequence Repeat (ISSR) DNA sequencing method. A total of 86.27% of polymorphic bands were seen in 94 reproducible bands generated from 12 number of ISSR primer ranging between 200bp to1000bp of band width. ISSR primer produces the bands with an average polymorphism of more than ninety nine percent among all the ecotypes. The dendrograms drawn for the analysis of genetic similarity in all the ecotypes. Our studies reveals that, genetic variations among different accessions of Gloriosa superba(L) was very well identified with the help of ISSR finger printings technic.\",\"PeriodicalId\":146947,\"journal\":{\"name\":\"Research in Plant Biology\",\"volume\":\"8 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Research in Plant Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.25081/RIPB.2018.V8.3600\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research in Plant Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.25081/RIPB.2018.V8.3600","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
INTRA SPECIFIC ANALYSIS OF GLORIOSA SUPERBA (L) THROUGH ISSR FINGER PRINTING AND DNA SEQUENCING OF ECOTYPES COLLECTED FROM DIFFERENT ACCESSIONS OF TAMIL NADU STATE, INDIA
Diversity within and among the population of Gloriosa superba collected from five different location of Tamil Nadu State, India, were explored by using Inter Simple Sequence Repeat (ISSR) DNA sequencing method. A total of 86.27% of polymorphic bands were seen in 94 reproducible bands generated from 12 number of ISSR primer ranging between 200bp to1000bp of band width. ISSR primer produces the bands with an average polymorphism of more than ninety nine percent among all the ecotypes. The dendrograms drawn for the analysis of genetic similarity in all the ecotypes. Our studies reveals that, genetic variations among different accessions of Gloriosa superba(L) was very well identified with the help of ISSR finger printings technic.
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