Neuroprotective effect of Cistus laurifolius on hydrogen peroxide-induced neurodegeneration in differentiated SH-SY5Y cells

Neuroprotective effect of Cistus laurifolius on hydrogen peroxide-induced neurodegeneration in differentiated SH-SY5Y cells Hamiyet ECIROGLU1*, Fatma YILDIZ1, Ersin YUCEL2 ORCID: 0000-0002-3555-3946; 0000-0002-9270-9062; 0000-0001-8274-7578 1Department of Medical Laboratory Techniques, Vocational School of Health Services, Alanya Alaaddin Keykubat University, Antalya, Turkey 2Department of Biology, Faculty of Science, Eskisehir Technical University, Eskişehir, Turkey (ORCID: 0000-0001-8274-7578) Abstract Neurodegeneration is an important finding of various neurological diseases such as Alzheimer's Disease and Parkinson's Disease. MAP2 and Rbfox3/NeuN genes are also two important neuronal markers. In this study, our aim is to investigate the neuroprotective effect of Cistus laurifolius L. extract in neurodegeneration caused by H2O2 treatment in differentiated SH-SY5Y (f-SH-SY5Y) cells. In the study, the effects of H2O2 (62.5-1000µM) and Cistus laurifolius (15.62-1000mg/ml) doses on cell viability were determined by the MTT method. The effect of Cistus laurifolius in d-SH-SY5Y cells followed by H2O2 treatment on cell viability was determined by the MTT test. The effect of the extract on MAP2 and Rbfox3/NeuN gene expressions in 24-hour periods was evaluated by the RT-qPCR method. According to our findings, Cistus laurifolius extract significantly reduced cell viability in SH-SY5Y cells at 24 h and 48 h (p