编码兔精子膜蛋白的cDNA (RSD-1)的核酸序列及其结构特性。

Proceedings of the Chinese Academy of Medical Sciences and the Peking Union Medical College = Chung-kuo i hsueh k'o hsueh yuan, Chung-kuo hsieh ho i k'o ta hsueh hsueh pao Pub Date : 1990-01-01

B Yun, S Miao, Y Yan, L Wang, S S Koide

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摘要

采用链终止法测定了兔精子尾膜蛋白2.0 kb cDNA的核苷酸序列。在M13mp18/19克隆系统中，利用Sequenase和Klenow酶对目标序列进行分析。开放阅读框包含438 bp，编码146个氨基酸，计算Mr为15507。疏水氨基酸含量为34.9%，pI在5.8附近。

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Nucleic acid sequence of cDNA (RSD-1) encoding a rabbit sperm membrane protein and structural properties of the encoded protein.

The nucleotide sequence of a 2.0 kb cDNA encoding a rabbit sperm tail membrane protein was determined using the chain termination method. Analysis was performed using Sequenase and Klenow enzymes on the target sequence in an M13mp18/19 cloning system. The open reading frame consisted of 438 bp encoding 146 amino acids of the protein, with a calculated Mr of 15507. The hydrophobic amino acid content was 34.9%, and pI was in the vicinity of 5.8.

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Proceedings of the Chinese Academy of Medical Sciences and the Peking Union Medical College = Chung-kuo i hsueh k'o hsueh yuan, Chung-kuo hsieh ho i k'o ta hsueh hsueh pao

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