采用FR4二色性扫描仪和大数值孔径大视角物镜的低成本介观镜

Naitao Xu, Jin Cheng, Wenjing Chen, Qiliang Sun, Weiguo Liu, Yingshun Xu
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引用次数: 0

摘要

在本文中,作者报告了一种低成本的介观镜,与传统的多光子显微镜相比,它具有高达3.4mm的大视场(FOV)和更紧凑的外形尺寸。它由一个两轴电磁驱动的FR4二向色扫描仪和定制物镜组成,同时具有高数值孔径(NA)(~0.7)和大视角(AOV)(30度)。FR4二向色扫描仪直接扫描定制物镜的后焦平面。低成本的中观镜可以有效地记录大面积的神经活动,并在低倍率下执行亚细胞分辨率图像。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Low Cost Mesoscope by a FR4 Dichroic Scanner and a High Numerical Aperture and Large Angle of View Objective Lens
In this article authors report a low cost mesoscope featuring its large field of view (FOV) up to 3.4mm and more compact outline dimensions in comparison with conventional multiphoton microscopes. It consists of a two-axis electromagnetic actuated FR4 dichroic scanner as well as customized objective lens with simultaneously a high numerical aperture (NA) (~0.7) and a large angle of view (AOV) (30 degrees). The FR4 dichroic scanner scans directly at the back focal plane of a customized objective lens. The low cost mesoscope allows efficiently recording neural activities in a large area and performs subcellular resolution images in low magnification.
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