bsa -糖缀合物是基于spr的聚糖生物传感器的理想构建模块

IF 8.2 1区化学 Q1 CHEMISTRY, ANALYTICAL

ACS Sensors Pub Date : 2016-12-30 DOI:10.1021/acssensors.6b00679

Shun Tao, Tian-Wei Jia, Yang Yang*, Li-Qiang Chu*

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引用次数: 14

摘要

糖探针的可控固定化是开发糖类生物传感器的关键。为此，通过方酸介导的偶联反应，制备了一系列具有不同甘露糖单位数的bsa -糖偶联物。该缀合物可以直接吸附在金底物上，不需要任何衍生反应，从而为构建基于spr的聚糖生物传感器提供了一种简单有效的方法。SPR测定结果表明，含有11种甘露糖的bsa -甘露糖偶联物对凝集素concanavalin A的亲和力最高，检测限约为1.8 nM。还研究了所获得的聚糖生物传感器的再生和特异性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

BSA-Sugar Conjugates as Ideal Building Blocks for SPR-Based Glycan Biosensors

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BSA-Sugar Conjugates as Ideal Building Blocks for SPR-Based Glycan Biosensors

Controlled immobilization of sugar probes is of key importance for the development of glycan biosensors. To this end, a series of BSA-sugar conjugates with different numbers of mannose units are prepared via the squaric acid-mediated coupling reaction. The conjugates can absorb directly on gold substrate without any derivation reactions, thus providing a simple and effective method for the construction of SPR-based glycan biosensors. SPR measurements show that the BSA-mannose conjugate with 11 mannoses exhibit the highest affinity to the lectin concanavalin A with a limit of detection of ca. 1.8 nM. Regeneration and specificity of the obtained glycan biosensors are also investigated.

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来源期刊

ACS Sensors Chemical Engineering-Bioengineering

CiteScore

14.50

自引率

3.40%

发文量

372

期刊介绍： ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.