C Farace, B Blanchot, D Champiat, P Couble, G Declercq, J L Millet
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引用次数: 6
摘要
以低收率合成了荧光素的衍生物4-甲基- d -荧光素，并对其紫外光谱和荧光光谱进行了表征。荧光素及其衍生物的光谱非常相似。4-甲基- d荧光素是pyralis Photinus荧光素酶的底物，在光度测定中引起发光。
Synthesis and characterization of a new substrate of Photinus pyralis luciferase: 4-methyl-D-luciferin.
A derivative of luciferin, 4-methyl-D-luciferin, was synthesized in low yield, and characterized by its ultraviolet and fluorescence spectra. The spectra of luciferin and its derivative are very similar. 4-Methyl-D-luciferin is a substrate of Photinus pyralis luciferase, and it causes light emission in luminometric assays.
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