采用固相竞争ELISA (SPCE)技术测定自然感染牛口蹄疫血清型

Muluneh Ayelech, Woldemariyam Fanos Tadesse
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摘要

目的:口蹄疫(FMD)是一种影响偶蹄家畜和野生动物的高传染性和重要的经济疾病。早期诊断和血清分型对有效设计和实施控制方法非常重要。本研究对2018年10月至2020年2月期间在阿姆哈拉、提格雷、奥罗米亚和亚的斯亚贝巴采集的血清样本进行了研究。这些动物在半集约化到粗放化的饲养体系下饲养。采用竞争NSP-ELISA法对OD值较低(阳性)的血清样品进行血清分型ELISA。结果:从186份NSP ELISA阳性血清中鉴定出3种血清型,其中O型156份,A型40份,SAT2型28份。在此分析中,观察到多种血清型感染,这就是为什么血清型数量超出分析样本的原因。在亚的斯亚贝巴的23份样本中,O型、A型和SAT2型分别为3份和5份,而在奥罗米亚地区的样本中,O型和SAT2型分别为12份和3份。在阿姆哈拉地区,经分析的99个样本在7个血清样本中发现血清O型和SAT2型。在提格雷地区,30个样本被发现为血清O型感染,其中13个为SAT2型。根据生产系统实践确定的血清型比例还发现,半集约型生产在所有三种血清型中所占比例最大,其次是粗放型生产。一般来说,早期确定过去感染的血清型有助于了解流行病学以及畜群/个体动物的感染免疫。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of foot-and-mouth disease serotypes from naturally infected cattle by solid phase competitive ELISA (SPCE) techniques
Objective: Foot and mouth disease (FMD) is a highly infectious and economically important disease affecting cloven-hoofed domestic and wild animals. Early diagnosis and serotyping of the agent are very important to effectively design and implement the control approach. This study was conducted on serum samples collected from Amhara, Tigray, Oromia and Addis Ababa between October 2018 to February 2020. The animals were kept under a semi-intensive to an extensive system of rearing. Serum samples with low OD values (positive) using competition NSP-ELISA were subjected to serotyping ELISA. Results: In the present study, three serotypes were identified from 186 NSP ELISA positive sera of which 156 serotype O, 40 serotypes A and 28 serotype SAT2. In this analysis, multiple serotype infection was observed which is why the number of serotypes was beyond the samples analyzed. Among 23 samples from Addis Ababa 10, 3 and 5 were O, A and SAT2 serotypes respectively, while in samples from the Oromia region 12 were O and 3 were SAT2 serotypes. From the Amhara region, 99 samples analyzed were found to be serotype O and SAT2 in 7 of the serum samples. From the Tigray region, 30 samples were seen to have Serotype O infection, whereas 13 of them were SAT2. The proportion of serotypes identified based on the production system practices was also found that semi-intensive production takes the largest share in all three serotypes followed by extensive production. Generally, early determination of the serotype from past infection helps to aware of the epidemiology as well as the infection immunity of the herd/individual animals.
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