CALLUS CULTURE AS THE METHOD IN PROVIDING ANTIMALARIAL COMPOUNDS OF PIPER GENUS

Malaria is still a serious cases all over the world especially in tropical country with a predict number 300-500 millions people infected per year. The resistence cases of these anti-malaria substance make a new effort to find the new strategy against malaria. This study aim to summarize the potential Piper genus as the source of potential antimalarial compound and recent research of callus induction in piper plant to obtain metabolites.The study of Piper genus represent the 11 Piper species were chemically studied and assayed against the plasmodium. The potential antimalarial compound isolates from several Piper genus were 20,60-Dihydroxy-40-methoxydihydro-chalcone,3-Farnesyl-p-hydroxybenzoic acid, Piperine, Chabamide, Benzoic acid derivatives, Guineensine, pellitorine, brachystamide B, sarmentine, and sermentosine, 5,8-Hydroxy-7-methoxyflavone, Prenylated hydroxybenzoic acid, 4-Nerolidylcatechol, Piperitone, Champor, and Viridiflorol (EO). An effort to propagate necessary antiplasmodial resources especially by callus induction has been conducted for 7 Piper species such as Piper betle, P. colubrinumm, P. crocatum, P. longum, P. nigrum, P. permucronatum and P. solmsianum to obtain higher content of secondary metabolites with a different plant growth hormone (PGR) supplementation while there are most of Piper genus have not been well studied. It conclude that callus culture could be the promising method to obtain antimalarial secondary metabolites as antimalarial.